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Isolation and characterization of dominant,pleiotropic drug-resistance mutants in Chlamydomonas reinhardtii
Authors:Steven W. James  Paul A. Lefebvre
Affiliation:(1) Department of Genetics and Cell Biology, University of Minnesota, 250 Biological Sciences Center, 1445 Gortner Avenue, 55108-1095 St. Paul, MN, USA;(2) Present address: Department of Pharmacology, UMDNJ-R. W. Johnson Medical School, 675 Hoes Lane, 08854 Piscataway, NJ, USA
Abstract:Summary Three independent pleiotropic drug-resistance (pdr) mutants were isolated by selecting for resistance to the anti-microtubule herbicides amiprophos-methyl (APM) and oryzalin (ORY). These three mutants and a previously isolated mutant, ani1 (anisomycin resistance), were semi-dominant in heterozygous diploids, and they displayed varying degrees of resistance to structurally and functionally unrelated inhibitors such as cycloheximide, cryptopleurine, emetine, atrazine, and nonidet P-40. Linkage analysis and genetic mapping suggested that three of the four mutants, including ani1, define a single locus, here named pdr1. The fourth mutant defined a new locus, pdr2, which is located on the left arm of linkage group VI. One pdr1 mutant exhibited unusual genetic interactions, including enhanced ts-lethality and synergistic increases in drug resistance, when combined with pdr2-1 and with herbicide-resistant alleles of three other genes.
Keywords:Chlamydomonas  Drug resistance  Pleiotropy  Dominance
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