| 白血病细胞膜相关因子（MAF－J6－1）单克隆抗体的制备及性质 |
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| 引用本文： | 耿以琪,饶青,孔健,杨丛林,李长虹,宋玉华,吴克复. 白血病细胞膜相关因子（MAF－J6－1）单克隆抗体的制备及性质[J]. 中华血液学杂志, 1994, 0(12) |
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| 作者姓名： | 耿以琪 饶青 孔健 杨丛林 李长虹 宋玉华 吴克复 |
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| 作者单位： | 天津中国医学科学院血液学研究所 |
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| 摘 要： | 用部分纯化的白血病细胞膜相关因子（ＭＡＦ－Ｊ６－１）对ＢＡＬＢ／Ｃ小鼠脾细胞进行体外免疫，然后与ＳＰ２／０小鼠骨髓瘤细胞融合获杂交瘤，经３次再克隆得到能产生ＭＡＦ－Ｊ６－１单克隆抗体（单抗）的杂交瘤细胞系。用ＡＢＣ免疫酶标法检测，该单抗在Ｊ６－１细胞上产生强阳性反应，并与ｒｈ－Ｍ－ＣＳＦ单抗对ＭＡＦ－Ｊ６－１及ｒｈ－Ｍ－ＣＳＦ产生交叉中和反应。ＭＡＦ－Ｊ６－１单抗还对Ｊ６－２、ＬＣＬ、Ｋ５６２等细胞系呈膜阳性反应，对白血病患者骨髓中部分原始和幼稚的单核、粒细胞呈阳性反应。在２４例被检测的白血病患者中，有三例呈强阳性反应，ＭＡＦ－Ｊ６－１可用作研究ｍ－Ｍ－ＣＳＦ与白血病关系的工具。
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| 关 键 词： | 白血病，膜相关因子，巨噬细胞集落刺激因子，抗体，单克隆 |
PREPARATION AND CHARACTERIZATION OF MoAb OFLEUKEMC CELL MEMBRANE ASSOCIATED FACTORMAF-J6-1 |
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| Geng Yiqi,Rao Qing,Kong Jian, et alInstuare of Hemaxology,Chinese Academy of MedicalSciences,Tianjin. PREPARATION AND CHARACTERIZATION OF MoAb OFLEUKEMC CELL MEMBRANE ASSOCIATED FACTORMAF-J6-1[J]. Chinese Journal of Hematology, 1994, 0(12) |
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| Authors: | Geng Yiqi Rao Qing Kong Jian et alInstuare of Hemaxology Chinese Academy of MedicalSciences Tianjin |
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| Affiliation: | Geng Yiqi,Rao Qing,Kong Jian, et alInstuare of Hemaxology,Chinese Academy of MedicalSciences,Tianjin,300020 |
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| Abstract: | BALB/ c mice splenocytes were immunized in vitrowith partialiy purified MAF-J6-1, and then fused withSP2/0 murine myeloma cells. After 3 times subcloning.a hybridoma cell lines secreting monorlonal antibody ofMAF-J6-1 was obtained. The MoAb showed strongpositive reaction with J6-1 cell membrane by ABC im-munoperoxidase staining, and cross neutralizations withM-CSF-MoAb to MAF-J6-1, rh-M-CSF on J6-1 cells.Human leukemic cell lines J6-2, LCL and K562 cellsshowed positive membrane reaction as well. On bonemarrow smears of leukemia patients , MAF-J6-1 MoAbbound to the membrane of monocytic lineage and somemyelocytes. MAF-J6-1-MoAb might be an useful toolfor studying the reiationship of m-M-CSF andleukemias. |
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| Keywords: | Leukemia Membrane associated fac-tor Macrophagecolony stimulatingfactor Monoclonal antibedy |
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