粗杂粮对大鼠脂代谢紊乱干预作用的机制探讨

目的观察粗杂粮对大鼠脂代谢紊乱及其脂肪组织过氧化物酶体增殖物激活受体γ (PPARγ) mRNA表达水平的影响,探讨粗杂粮改善脂代谢紊乱的作用机制。方法44只SPF级大鼠随机分为阴性对照组(饲常规饲料)和3个实验组(饲高脂饲料6周),造模成功后分别给予高脂粗杂粮、高脂米面和高脂模型饲料共15周。结果粗杂粮高脂组的血清总胆固醇(TC)、总甘油三酯(TG)、白细胞介素-6(IL-6)和C反应蛋白(CRP)显著低于高脂模型组(P<0.05),血清高密度脂蛋白胆固醇(HDL-C)水平显著高于高脂模型和米面高脂组(P<0.05),脂蛋白酯酶(LPL)和肝酯酶(HL)活性及肿瘤坏死因子-α(TNF-α)最接近阴性对照组水平;粗杂粮高脂组白色脂肪组织PPARγ mRNA的表达水平明显高于高脂模型组和米面高脂组(P<0.05)。结论复配式粗杂粮可以激活PPARγ,促进脂肪细胞LPL和HL活性逐步恢复,使血脂水平下降;同时抑制和减少IL-6、TNF-α及CRP的产生,炎症反应减轻,脂代谢紊乱得到改善。

Intervention of coarse cereals on lipid metabolism in rats

Objective To observe the effect of coarse cereals on improving the disorder of lipid metabolism and the expression of PPARγ mRNA in white adipose tissue in rats to investigate the mechanism of coarse cereals on lipid metabolism disorder. Methods Forty four SPF rats were randomly divided into 4 groups: the negative control group was fed with normal diet and 3 experimental groups were fed with high-fat modeling diet for 6 weeks for model building. The 3 experimental groups,the coarse cereals group,rice-flour group and the hyperlipemia model group, were then fed with coarse cereals high-fat diet,rice-flour high-diet and high-fat modeling diet respectively for another 15 weeks. Results Compared with the hyperlipemia modeling group,serum TG,TC,IL-6 and TNF-α in the coarse cereals group were declined significantly(P < 0. 05),serum HDL-C in coarse cereals group was higher than that in rice-flour group and hyperlipemia model group(P < 0. 05),LPL,HL and TNF-α in coarse cereal group were close to the negative control group. Moreover,the expression of PPARγ mRNA in white adipose tissue of the coarse cereals group was higher than other groups. Conclusion The coarse cereals could activate PPARγ and enhance the activity of key enzymes in lipids metabolism,so as to reduce the level of TG relieve inflammation and improve lipid dysmetabolism eventually.