中国南方部分城市泌尿生殖道沙眼衣原体基因分型研究

目的 了解中国南方4个城市性病高危人群中沙眼衣原体(Ct)基因型的分布特征。方法 从衡阳、上海、广州、江门四个城市的性病门诊收集1180份泌尿生殖道标本,经质粒PCR筛选后,阳性者用巢式PCR扩增Ctomp1基因片段,酶切omp1扩增产物,分析Ct各临床株omp1基因的限制性片段长度多态性(RFLP),将临床株分型。结果 Ct质粒PCR阳性者301份,用于分型者286份。通过RFLP分型,共检出10个基因型,其中常见的是E(30.8%)、J(23.8%)、D(17.5%)、F(10.5%)、G(6.2%)型,并且不同城市间基因型的分布差异无统计学意义(χ²=0.981,P>0.05)。结论 4个城市存在Ct多种基因型,并且基因型的分布大致相同。

Genotyping of Chlamydia trachomatis isolated from patients with sexually transmitted diseases from four cities in Southern China

Objective To investigate the distribution of Chlamydia trachomatis genotypes in high risk populations of STDs from four cities in Southern China.Methods C.trachomatis was detected by a plasmid PCR in 1180 specimens collected in Hengyang,Shanghai,Guangzhou and Jiangmen,and then the major outer membrane protein gene (ompl) was amplified by nested PCR (nPCR) with plas- mid-PCR-positive specimens.The amplicons of ompl were digested by restriction endonuclease Alu I,Msp I,EcoR I,or Dde I.C.trachoraatis was genotyped according to the restriction fragment length polymorphism (RFLP) patterns.Results Of 301 plasmid-PCR-positive specimens,286 (48 collected from Hengyang,32 from Shanghai,52 from Guangzhou and 154 from Jiangmen) could be genotyped.The most prevalent geno- type was serotype E(30.8%),followed by J(23.8%),D(17.5%),F(10.5%),and G (6.2%).The dis- tribution of various genotypes was not different among different cities.Conclusion Polymorphic genotypes of C.trachomatis are prevalent in Hengyang,Shanghai,Guangzhou and Jiangmen,and there is no significant difference of genotype distribution among these four cities.