| Abstract: | AIM: To investigate the effects of human induced pluripotent stem cell-derived exosomes( hi PSC-exo) on cell viability,capillarylike structure formation,and senescence in endothelial cells exposed to high glucose. METHODS: Exosomes were isolated from the conditional medium of hi PSCs and confirmed by transmission electron microscopy,nanoparticle tracking analysis,and Western blot analysis using Alix and CD63 as markers. hi PSC-exo were labeled with PKH26 for tracking. Cultured HUVECs were treated with high glucose(33 mmol / L) with or without hi PSC-exo(20 mg / L) for 48 h,and cell viability,capillary tube formation,and senescence were assessed. RESULTS: hi PSC-exo showed a typical cup shape and could be taken up by human umbilical vascular endothelial cells(HUVECs) in a concentration-dependent manner. When exposed to high glucose,viability and tube formation in HUVECs was significantly reduced,whereas the proportion of senescent cells was higher compared to that in control HUVECs( P 0. 01). Furthermore,hi PSC-exo restored cell viability and capillary-like structure formation,and reduced senescence in HUVECs exposed to high glucose(P 0. 01). However,hi PSC-exo had minimal effects on normal HUVECs. Therefore,stem cell-derived exosomes can promote cell proliferation, enhance capillary-like structure formation, and reduce senescence in endothelial cells exposed to high glucose.CONCLUSION: Our study highlights the role of exosomes derived from hi PSC and may provide a new strategy for maintaining vascular health,preventing vascular aging,and avoiding pathological vascular remodeling that occurs in many diseases. |
