HPLC测定白加黑中盐酸伪麻黄碱和氢溴酸右美沙芬的含量

 目的:测定白加黑中盐酸伪麻黄碱和氢溴酸右美沙的含量?方法:采用苯基键合硅胶柱;流动相为乙腈-水-磷酸(40∶60∶0.1),其中每1000ml该溶液中加入十二烷基硫酸钠(SDS)4.0g;以盐酸萘甲唑啉为内标;检测波长为224nm;流速为1.0ml·min^-1?结果:盐酸伪麻黄碱线性范围0.225～1.125mg·ml^-1,平均回收率为100.23%,RSD=0.69%(n=7);氢溴酸右美沙芬线性范围0.112～0.560mg·ml^-1,平均回收率为99.81%,RSD=0.95%,(n=7)?结论:方法简便,结果准确?

Determination of pseudoephedrine hydrochloride and dextromethorphan hydrobromide in baijiahei by HPLC

OBJECTIVE:To develop a HPLC method for determination of pseudoephedrine hydrochloride and dextromethorphan hydrobromide in Baijiahei.METHODS:Chromatographic conditions included Nova-pak Phenyl column and the mobile phase consisting of a minxture of acetomitrile-water-phosphoric acid (40∶60∶0.1).There are four grams dodecylsulfate Na per litre of the mobile phase.Naphazoline hydrochloride was selected as the internal standard.Detection wavelength was at 224nm.The flow rate was 1ml·min^-1.RESULTS:The standard curve of Pseudoephedrine hydrochloride was linear ever the range of 0.225～1.125mg·ml^-1.The average recovery was 100.23% withi RSD=0.69%(n=7).The standard curve of dextromethorphan hydrobromide was linear over the range of 0.112～0.560mg·ml^-1.The average recovery was 99.81% with RSD=0.95% (n=7).CONCLUSION:This method was found to be simple and accurate.