IL-6、IL-8 及 MMP-9 在胎膜早破者血清、羊水中的含量及意义简

目的 探讨孕妇血清、羊水中白细胞介素-6（IL-6）、白细胞介素-8（IL-8）、基质金属蛋白酶-9（MMP-9）对胎膜早破、宫内感染的临床意义.方法 某院产科住院分娩的胎膜早破孕妇100例为研究组,对照组为同期住院无妊娠并发症剖宫产的孕妇100例.采用酶联免疫吸附法检测IL-6、IL-8及MMP-9在孕妇血清、羊水中的含量.同时产后取胎膜送病理检查.结果 研究组孕妇血清、羊水中IL-6、IL-8及MMP-9的含量均高于对照组,差异有统计学意义（P〈0.05）;宫内感染者孕妇血清、羊水中IL-6、IL-8及MMP-9水平高于非宫内感染者,差异有统计学意义（P〈0.05）.随着破膜时间延长,孕妇血清、羊水中IL-6、IL-8及MMP-9的含量增高.结论 IL-6、IL-8及MMP-9联合检测可用于胎膜早破及宫内感染的早期诊断,并指导临床用药,对确定终止妊娠的最佳时机也具有一定的临床意义.

The levels and significance of IL-6,IL-8 and MMP-9 in serum and amniotic fluid of premature rupture of membranes

Objective To investigate the clinical significance of interleukin-6 （ IL-6）, interleukin-8 （ IL-8）, matrix metropolitanate-9 （MMP-9） in maternal serum and amniotic fluid of pregnant women with premature rupture of membranes and intrauterine infection. Methods The levels of IL-6,IL-8 and MMP-9 in maternal serum and amniotic fluid were detected in one hundred cases with premature rupture of membranes （ prom group） and one hundred cases of cesarean section without pregnancy complications （ control group） by enzyme-linked immunosorbent assay （ELISA）. Two hundred cases were enrolled from the department of obstetric in the third affiliated a hospital, and postnatal fetal membranes required for pathological examination. Results The levels of IL-6, IL-8 and MMP-9 in prom group were higher than those in control group （ P 〈 0.05 ）. The levels of IL-6, IL-8 and MMP-9 in maternal serum and amniotic fluid with Intrauterine infection were higher than non-intrauterine infection （ P 〈 0. 05 ）. With prolonged rupture of membranes, the levels of IL-6,IL-8 and MMP-9 gradually increased. Conclusion The combined detection of IL-6,1L-8 and MMP-9 can be used for the early diagnosis of premature rupture of membranes and intrauterine infection, and to guide the clinical medication, also has certain clinical significance to determine the optimal timing of termination of pregnancy.