大鼠血清培养液对食管癌细胞增殖影响

目的筛选食管癌细胞增殖培养液中大鼠血清的最佳浓度和活性状态。方法采用常规饲料喂饲大鼠30d,股动脉采血制备血清,采用不同浓度和活性状态的大鼠血清培养液培养人食管上皮鳞癌细胞株Eca-109,采用四甲基偶氮噻唑蓝(MTT)法,筛选大鼠血清培养液对Eca-109细胞生长增殖的最佳作用条件,以小牛血清培养液及人正常肝上皮细胞株HL7702作为对照进行比较。结果各浓度未灭活大鼠血清培养的人食管癌细胞Eca-109基本上均呈指数增长趋势,72h时各浓度组间差异有统计学意义(F=159.263,P<0.05);用5%未灭活大鼠血清培养人食管癌细胞Eca-109和人正常肝上皮细胞HL7702,培养72h时,2株细胞的生长增殖能力均强于5%灭活大鼠血清,差异均有统计学意义(F=37.746,P<0.05;F=43.399,P<0.05);与小牛血清对2株细胞生长增殖的作用接近(P>>0.05)。结论5%相同浓度条件下,未灭活大鼠血清培养液更适合细胞生长。

Effects of medium with serum of rat on proliferation of esophageal cancer cell line

ObjectiveTo determine the optimal concentration and activity of rat serum in the medium for cultivating human esophageal cancer cell line.MethodsTwenty SD rats were fed with the standard diet for 30 days and then sacrificed via femoral artery bleeding and the sera were separated and collected.The sera of different concentration and activities were added into the medium for esophageal cancer cell Eca-109 culture.Methyl thiazolyl tetrazolium(MTT)assay was used to determine the optimal conditions of the sera for cultivating Eca-109 cell.Fetal bovine serum(FBS)at concentration of 10% and human normal liver epithelial cell line HL7702 were set as the serum control and the normal cell line control,respectively.ResultsUn-deactivated sera at different concentrations made Eca-109 cells grow with a exponential trend and there were significant differences in the growth of the cells between different groups at 72hr.Un-deactivated serum provided better condition for culturing Eca-109 cell and HL7702 cell than deactivated serum at the same concentration of 5% at 72hr.The serum showed the same effects on the growth of the two cell lines as the FBS.ConclusionUn-deactivated rats'serum is more suitable for cultivating Eca-109 cells than the deactivated serum at the same concentration of 5%.