人血浆HDL对高脂饲养家兔肝细胞膜HDL受体活性的影响

为进一步探讨ＨＤＬ对抗动脉粥样硬化的作用机理，采用高胆固醇膳食饲养家兔，通过静脉注射人血浆ＨＤＬ制剂，观察ＨＤＬ对高脂饲养家兔血脂、肝脏和胆囊胆汁脂质含量以及肝细胞膜ＨＤＬ受体活性的影响。结果发现：ＨＤＬ制剂虽无降低高脂饲养家兔血清脂质的作用，但可减少其肝脏脂质沉积，并且具有促进脂质经胆道排泄的作用。高脂饲养家兔的肝细胞膜ＨＤＬ受体具有Ｋｄ 值减小，Ｂｍ ａｘ 值增加的趋势；注射ＨＤＬ制剂的家兔，与正常对照比较，其Ｋｄ 值虽无明显变化，但Ｂｍ ａｘ 值显著增加（Ｐ＜ ０．０５），表明注射人血浆ＨＤＬ可使高脂饲养家兔肝细胞膜ＨＤＬ受体呈现以受体数目增加为特征的活性升高。

Effect of Human Plasma HDL on the HDL Receptors of Plasma Membranes of Cholesterol-fed Rabbit

Atherosclerosis(As) rabbit model was developed by high cholesterol feeding for 12 weeks.The rabbits were injected intravenously with human plasma HDL preparation per week, and then the effects of HDL on the lipids contents of serum,liver and bile nd the activity of HDL receptors on liver plasma membranes of cholesterol fed rabbit were investigated.The results showed that HDL preparation had no effect on decreasing the lipids contents of serum,but it could low down the lipid depositions in liver,and promote the excretion of lipids from bile.The value of Bmax of HDL receptor showed decreasing trendy and the value of Kd showed increasing trendy in cholesterol fed rabbits.In HDL treated rabbits,the value of Bmax increased significantly as compared with that of normal group( P <0.05),but the value of Kd showed no difference.The results suggested that human plasma HDL could enhance the activity of HDL receptors on the liver plasma membranes of cholesterol fed rabbit.