氯沙坦对血管平滑肌细胞富含半胱氨酸蛋白61的影响

目的探讨氯沙坦对血管平滑肌细胞富含半胱氨酸蛋白61(CYR61)的影响。方法贴块法培养大鼠血管平滑肌细胞,分别给予不同浓度血管紧张肽Ⅱ(ATⅡ)和氯沙坦,采用半定量逆转录聚合酶链反应及Western blotting检测不同处理组平滑肌细胞CYR61 mRNA和蛋白表达。采用酶联免疫吸附技术检测平滑肌细胞培养介质中CYR61含量的变化。结果10~(-6)/10~(10) mol·L~(-1)ATⅡ呈剂量依赖性地增加平滑肌细胞内CYR61蛋白及其mRNA表达水平,增高培养介质中CYR61含量(P<0.01)。10~(-5)～10~(-7)mol·L~(-1)氯沙坦预处理组中,ATⅡ刺激的平滑肌细胞CYR61蛋白及其mRNA表达水平及培养介质中CYR61含量明显减低(P<0.01)。结论氯沙坦可拮抗ATⅡ所致的平滑肌细胞内CYR61的表达与分泌。

Effects of losartan on expression of cysteine-rich 61 in cultured rat vascular smooth muscle cells

AIM To investigate the effects of losartan on the expression of cysteine-rich 61(CYR61)in cultured rat vascular smooth muscle cells(VSMCs).METHODS The VSMCs were cultured by the primary- explant methods.The levels of protein and mRNA of CYR61 in cultured VSMCs and the contents of CYR61 in the media were detected by RT-PCR,Western blotting and enzyme-linked immunosorbent assay.RESULTS 10~(-6)-10~(-10) mol·L~(-1) angiotensinⅡ(ATⅡ)increased the levels of protein and mRNA of CYR61 in the cultured VSMCs and the contents of CYR61 in the media in a dose-dependent manner(P<0.01).10~(-5)-10~(-7) mol·L~(-1) losartan markedly reduced the protein and mRNA lever of CYR61 in the cultured VSMCs and the contents of CYR61 in the media induced by ATⅡ.CONCLUSION Losartan may antagonize ATⅡ-induced protein and mRNA expression and the secretion of CYR61 in the cultured VSMCs,which might play an important role in preventing the atherosclerosis and the restenosis after angioplasty.