消癌平辅助丝裂霉素对肺癌细胞化疗增效的研究

目的：通过消癌平联合丝裂霉素对肺癌 A549细胞增殖，细胞周期、凋亡及相关基因表达的影响，探讨化疗增效机制。方法MTT 检测消癌平（2、8、21μl／ml）联合丝裂霉素（2μg／nl）对肺癌 A549细胞生长抑制（联合用药 A、B、C 组），并设立对照组、丝裂霉素组和消癌平组；流式细胞术检测细胞周期及凋亡；RT－PCR 检测 P53、CyclinD1基因表达。结果MTT 检测显示在24、48、72 h 联合用药 A、B、C 组 OD（光密度）均比丝裂霉素低（ P ＜0．05）；流式细胞术检测表明消癌平、丝裂霉素与对照组相比，肺癌细胞均阻滞于 G0／G1期，差异有统计学意义（ P ＜0．05）。联合用药组 G0／G1期细胞比率、细胞凋亡率与丝裂霉素组比较，差异有统计学意义（ P ＜0．05）。消癌平组、丝裂霉素组与对照组比较，均能提高 P53 mRNA 表达（ P ＜0．05），联合治疗组 P53 mRNA 表达高于丝裂霉素组（ P ＜0．05）。消癌平组、丝裂霉素组与对照组比较均能降低 Cyclin D1基因的表达（ P ＜0．05）。与丝裂霉素组比较，联合用药可显著减低CyclinD1基因的表达（ P ＜0．05）。结论消癌平辅助丝裂霉素对肺癌细胞化疗增效可能与上调 P53 mRNA 表达，增强细胞凋亡作用，以及下调 CyclinD1 mRNA 表达，阻滞细胞周期于 G0／G1期有关。

Effects of Xiaoaiping injection combined with mitomycin on lung cancer cells in vitro

Objective To observe the effects of Xiaoaiping injection combined with mitomycin on cell proliferation, cell cycle, cell apoptosis and expressions of related genes (CyclinD1,P53 mRNA) of lung cancer-A549 cells in vitro,and to explore potential action mechanism.Methods The inhibitory effects of Xiaoaiping injection (2,8,21μl/ml) combined with mitomycin (2μg/nl) were detected by MTT (combination treatment group A,B,C),moreover, control group, Xiaoaiping group and mitomycin group were established.The cell cycle and cell apoptosis of lung cancer-A549 cells in vitro were detected by cytometry,and the expression levels of CyclinD1 and P53 mRNA were measured by RT-PCR.Results The results by MTT showed that the optical density value (OD) on 24h,48h,72h in combination treatment group A,B,C was significantly higher than that in mitomycin group ( P <0.05).The results by cytometry showed that as compared with control group,the lung cancer cells were blocked at G0/G1 phase in Xiaoaiping group and mitomycin group ( P <0.05).There were significant differences in the cell ratio at G0 /G1 phase and cell apoptosis rate between combination treatment groups and mitomycin group ( P <0.05).The expression levels of P53 mRNA in Xiaoaiping group and mitomycin group were significantly increased,as compared with those in control group ( P <0.05).Moreover the expression levels of P53 mRNA in combination treatment groups were significantly higher than those in mitomycin group ( P <0.05).The expression levels of Cyclin D1 in Xiaoaiping group and mitomycin group were significantly decreased,as compared with those in control group ( P <0.05).Furthermore the expression levels of Cyclin D1 in combination treatment groups were significantly decreased,as compared with those in mitomycin group ( P <0.05).Conclusion Xiaoaiping injection combined with mitomycin can enhance the efficiency of chemotherapy on lung cancer-A549 cells in vitro,and the action mechanism may be correlated to up -regulating the expression levels of P53 mRNA,increasing cell apoptosis and down -regulating the expressions of CyclinD1 mRNA to block cancer cells at G0/G1 phase.