广东紫珠中黄酮碳苷的分离与结构鉴定及初步体外抗炎活性研究

利用AB-8大孔树脂、Sephadex LH-20、C18及硅胶等柱色谱法分离纯化了广东紫珠的抗炎活性部位。根据理化性质和波谱方法(1H NMR、13C NMR、HMBC、ESI-MS等)鉴定化合物的结构。结果从中分离鉴定了5个黄酮碳苷化合物,分别为5,7,3’,4’-四羟基-8-C-β-D-葡萄糖黄酮碳苷(Ⅰ)、5,7,3’,4’-四羟基-6-C-α-L-鼠李糖-(1→2)]-β-D-葡萄糖黄酮碳苷(Ⅱ)、5,7,3’,4v-四羟基-8-甲氧基-6-C-β-D-葡萄糖黄酮碳苷(Ⅲ)、5,4’-二羟基-6,7-二甲氧基-8-C-β-D-木糖-(1→2)]-β-D-葡萄糖黄酮碳苷(Ⅳ)及5,4’-二羟基-6,7-二甲氧基-8-C-β-D-葡萄糖黄酮碳苷(Ⅴ)。化合物Ⅰ为首次从该植物中分离得到,其余4个化合物均为首次从紫珠属植物中分离得到。考察了5个化合物的抗环氧合酶-2活性,结果化合物Ⅰ、Ⅱ、Ⅲ均显示较强的体外抗炎活性。

Isolation and Structural Identification of C-Glycosylflavones from Callicarpa kwangtungensis Chun. and Their Preliminary Anti-inflammatory Activities

The anti-inflammatory activity fractions were isolated from Callicarpa kwangtungensis Chun.and purified by column chromatographies,such as AD-8 macroporous resin,Sephadex LH-20,C18 and silica gel.Their structures were identified on the basis of physiochemical properties and spectroscopic methods(1H NMR,13C NMR, HMBC and ESI-MS).Five C-glycosylflavones were isolated and identified as 5,7,3’,4’-tetrahydroxy-8-C-β-D-glucopyranosyl flavone(Ⅰ),5,7,3’,4’-tetrahydroxy-6-C-α-L-rhamnopyranosyl-(1→2)]-β-D-glucopyranosyl flavone(Ⅱ),5,7,3’,4’-tetrahydroxy-8-methoxy-6-C-β-D-glucopyranosyl flavone(Ⅲ),5,4’-dihydroxy-6,7-dimethoxy-8-C-β-D-xylopyranosyl-(1→2)]-β-D-glucopyranosyl flavone(Ⅳ) and 5,4’-dihydroxy-6,7-dimethoxy-8-C-β-D-glucopyranosyl flavone(Ⅴ).CompoundⅠwas isolated from Callicarpa kwangtungensis Chun.for the first time while the others were found from Callicarpa for the first time.The preliminary anti-inflammatory activities of the five compounds were investigated.The results showed that compounds Ⅰ,Ⅱ and Ⅲ had significant in vitro anti-inflammatory activity.