| 糖肽类抗生素A40926B产生菌的培养基优化及菌种选育 |
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| 引用本文： | 黄灵丽,沈晓放,陈少欣.糖肽类抗生素A40926B产生菌的培养基优化及菌种选育[J].中国医药工业杂志,2012,43(4):256-259. |
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| 作者姓名： | 黄灵丽 沈晓放 陈少欣 |
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| 作者单位： | 中国医药工业研究总院上海医院工业研究院,创新药物与制药工艺国家重点实验室,上海200040 |
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| 基金项目： | 微生物药物技术创新与新药创制产学研联盟 |
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| 摘 要： | 以A40926 B产生菌野野村放线菌SIPI-D-30为出发菌株,通过UV诱变及乙酸钠和甘氨酸抗性平板筛选,获得A40926 B产量提高的突变株SIPI-U-157。经优化,确定摇瓶发酵培养基(g/L)为:葡萄糖5.0、麦芽糊精30.0、可溶性淀粉10.0、玉米淀粉30.0、大豆蛋白胨5.0、肉蛋白胨10.0、酵母浸粉5.0、冷榨黄豆饼粉10.0、棉籽饼粉5.0、L-缬氨酸1.0,发酵培养7 d,突变株的A40926 B发酵单位为893 mg/L。该突变株在5 L发酵罐中培养180 h,A40926 B的产量最高为583 mg/L。
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| 关 键 词： | 野野村放线菌 A40926 菌种选育 发酵 |
Optimization of Fermentation Medium and Mutation Breeding of A Glycopeptide Antibiotic A40926 B Producing Strain |
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| Authors: | HUANG Lingli SHEN Xiaofang CHEN Shaoxin |
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| Institution: | *(State Key Lab.of New Drug & Pharmaceutical Process,Shanghai Institute of Pharmaceutical Industry,China State Institute of Pharmaceutical Industry,Shanghai 200040) |
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| Abstract: | A medium for Nonomuraea sp.SIPI-D-30 fermentation was developed and the productivity of glycopeptide A40926 B was improved by strain breeding.After SIPI-D-30 was treated with UV and screened in the resistant plate of sodium acetate and glycin,a mutant SIPI-U-157 with higher production of A40926 B was obtained.The effects of carbon and nitrogen sources on the antibiotic production in shake-flask culture were investigated.The optimized medium compositions(g/L) were glucose 5.0,maltodextrin 30.0,soluble starch 10.0,corn starch 30.0,soy peptone 5.0,meat peptone 10.0,yeast extract 5.0,soybean meal 10.0,cotton seed meal 5.0 and L-valine 1.0,in which the production of A40926 B by strain SIPI-U-157 reached 893 mg/L after 7 days’ cultivation.In addition,a fed-batch culture of SIPI-U-157 in a 5 L fermentator also conducted.The yield of A40926 B was 583 mg/L at 180 h. |
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| Keywords: | Nonomuraea sp A40926 strain breeding fermentation |
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