| 野百合碱致右心衰竭幼鼠Connexin43的变化及卡托普利和坎地沙坦的干预作用 |
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| 引用本文： | 张军毅,安金斗,梁芳,葛薇,冯嵩. 野百合碱致右心衰竭幼鼠Connexin43的变化及卡托普利和坎地沙坦的干预作用[J]. 实用儿科临床杂志, 2011, 26(1): 28-31 |
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| 作者姓名： | 张军毅 安金斗 梁芳 葛薇 冯嵩 |
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| 作者单位： | 1. 郑州大学第一附属医院,儿内科,郑州,450052
2. 江门市中心医院,儿内科,广东,江门,529000 |
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| 基金项目： | 河南省医学科技攻关和临床应用技术研究项目(200716602) |
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| 摘 要： | 目的 探讨Connexin43(Cx43)在野百合碱(MCT)致右心衰竭幼鼠中的变化及卡托普利和坎地沙坦的干预作用.方法 50只SD幼鼠腹腔注射MCT诱发肺动脉高压(PAH),随机分为心衰4周组(E1组)、心衰6周组(E2组)、卡托普利组(C1组)、坎地沙坦组(C2组)和二药联合组(C3组).另20只幼鼠腹腔注射9 g·L-1盐水作为对照,分为正常对照4周组(N1组)和正常对照6周组(N2组).测定每组存活幼鼠的血流动力学和相关生理指标,采用ELISA法检测其血清Cx43水平,免疫组织化学法和 RT-PCR法测定其右心室心肌组织Cx43和Cx43 mRNA的表达.结果 E1组与N1组比较,右心室肥厚指数、中心静脉压(CVP)、右心室收缩压 (RVSP) 显著升高,右心室压最大上升速率 (RVdp/dt) 显著降低,右心室心肌细胞排列紊乱、肌浆溶解,并可见空泡变性,右心衰竭模型成功.E1组血清Cx43水平、右心室心肌组织Cx43和Cx43 mRNA均较N1组降低.E2组与N2组比较,CVP、RVSP显著增高,RVdp/dt显著降低,血清Cx43水平、右心室心肌组织Cx43蛋白和Cx43 mRNA均降低.E2组与E1组比较,右心室肥厚指数、CVP、RVSP持续增高,RVdp/dt持续降低,E2组血清Cx43、右心肌组织Cx43蛋白和Cx43 mRNA进一步降低.C1组、C2组与E2组比较,血流动力学改善,右心室肥厚减轻,血清Cx43水平、右心肌组织Cx43蛋白和Cx43 mRNA均增高.C1组、C2组分别与C3组比较,血流动力学、血清Cx43水平、右心室心肌组织Cx43蛋白和Cx43 mRNA表达比较差异均无统计学意义.结论 PAH致右心衰竭时,Cx43参与心力衰竭的发生发展,随着心力衰竭的加重,其表达进一步降低;Cx43在幼鼠血清、右心室心肌组织中的水平具有一致性;卡托普利或坎地沙坦可能通过促进Cx43的表达缓解压力负荷性右心衰竭,二者比较无明显差异,联合用药并不能提高疗效.
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| 关 键 词： | 右心衰竭 Connexin43 卡托普利 坎地沙坦 野百合碱 幼鼠 |
Changes of Connexin43 in Juvenile Rats with Right Heart Failure Induced by Monocrotaline and Intervention Effects of Captopril and Candesartan |
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| ZHANG Jun-yi , AN Jin-dou , LIANG Fang , GE Wei , FENG Song. Changes of Connexin43 in Juvenile Rats with Right Heart Failure Induced by Monocrotaline and Intervention Effects of Captopril and Candesartan[J]. Journal of Applied Clinical Pediatrics, 2011, 26(1): 28-31 |
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| Authors: | ZHANG Jun-yi AN Jin-dou LIANG Fang GE Wei FENG Song |
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| Affiliation: | ZHANG Jun-yi1,AN Jin-dou1,LIANG Fang2,GE Wei1,FENG Song1(1.Department of Pediatrics,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,Henan Province,China,2.Department of Pediatrics,the Central Hospital of Jiangmen,Jiangmen 529000,Guangdong Province,China) |
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| Abstract: | Objective To explore the changes of connexin43(Cx43) in serum and its expression in right ventricular myocardium of juvenile rats with right heart failure induced by monocrotaline(MCT) and the intervention effects of captopril and candesartan on them.Me-thods Fifty juvenile SD rats were injected in belly cavity with MCT to induce pulmonary artery hypertension(PAH),and they were randomly divided into 5 groups:heart failure 4 weeks group(group E1),heart failure 6 weeks group(group E2),captopril group(group C1... |
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| Keywords: | right heart failure Connexin43 captopril candesartan monocrotaline juvenile rat |
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