IL-8基因多态性与慢性HBV感染结局的相关研究

目的研究IL-8基因启动子区-251A/T多态性及血清IL-8水平与慢性HBV感染结局的相关性。方法采用聚合酶链反应-限制性片段长度多态性技术（PCR-RFLP）检测60例慢性HBV感染者（其中轻度感染25例,中度17例,重度18例）和40例急性自限性HBV感染者（对照组）IL-8基因启动子区-251A/T位点多态性,同时采用酶联免疫吸附试验（ELISA）检测各组血清中IL-8的水平。结果①IL-8基因启动子区-251A/T多态性中各基因组频率（AA、AT、TT）和等位基因频率在病例组与对照组相比,差异均有统计学意义（P=0.002,0.000）。相对危险度分析,A等位基因携带者患慢性HBV感染的风险性是T等位基因的3.080倍（OR=3.080,95%CI：1.697-5.590）。②IL-8基因启动子区-251A/T多态性中各基因型和等位基因频率在慢性HBV感染轻度、中度和重度差异无统计学意义（P=0.463,0.209）。③慢性HBV感染者血清中IL-8水平显著高于对照组（P〈0.01）,且在慢性HBV感染轻度、中度和重度三组之间,IL-8的水平差异有统计学意义（P〈0.01）。结论 IL-8基因启动子区-251A/T与慢性HBV感染的发病相关,但与疾病的严重程度无明显的关系;A等位基因可能是慢性乙肝发生的易感基因之一;慢性HBV感染患者血清IL-8水平明显增高,且与病情的严重程度相关。

Association of Interleukin-8 Gene Polymorphisms and Its Serum Levels with Chronic HBV Infection

Objective To investigate the relationship between the gene polymorphism of interleukin-8-251A/T in the gene promoter and its serum levels with chronic HBV infection. Methods Polymorphisms of interleukin-8 gene were typed using polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） in 60 chronic HBV infection and 40 acute self-limit- ed infection. The serum interleukin-8 levels were measured enzyme-linked immunosorbent assay（ELISA）. Results ①There was significant difference in frequencies of allele and genotype in interleukin-8-251A/T polymorphism between chronic HBV infection and controls （ P = 0. 002, 0. 000 ）. Relative risk analysis indicated that the risk of chronic HBV infection in A allele was 3. 080 times higher than that in T allele （ OR = 3. 080,95 % CI ： 1. 697-5. 590）. ②The frequencies of genotypes and allele of IL-8-251 A/ T were found no significant difference between chronic HBV infection mild degree, moderate degree and severe degree （ P = 0. 463,0.209 ）. ③erum levels of interleukin-8 were significantly increased in chronic HBV infection compared with controls （P〈0.01）, the serum levels of interleukin-8 were found significant difference between chronic HBV infection mild degree group, moderate degree group and severe degree group （ P 〈 0.01 ）. Conclusion Interleukin-8-251A/T polymorphism was associ- ated with chronic HBV infection, and A allele might be a risk factor for chronic HBV infection, but there were no associations among three groups ; The level of serum interleukin-8 was increased in chronic HBV infection, which was correlated with illness se-