| ＩＬ—６在Ｕ９３７细胞中特异、持续激活ＳＴＡＴ３ |
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| 引用本文： | 张纪岩,黎燕,沈倍奋. ＩＬ—６在Ｕ９３７细胞中特异、持续激活ＳＴＡＴ３[J]. 细胞与分子免疫学杂志, 2001, 17(1): 16-19 |
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| 作者姓名： | 张纪岩 黎燕 沈倍奋 |
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| 作者单位： | 军事医学科学院基础医学研究所, |
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| 基金项目： | 国家杰出青年基金资助,No.39925019 |
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| 摘 要： | 目的 探讨ＩＬ－６在Ｕ９３７细胞中产生生物学效应的信号转导基础。方法 检测ＩＬ－６对Ｕ９３７细胞生长与分化的影响。并采用凝胶阻滞电泳法，检测ＩＬ－６在Ｕ９３７细胞中激活核因子的情况。结果 ＩＬ－６可诱导Ｕ９３７细胞向巨噬细胞方向分化。分化的细胞酸性醋酸酯酶（ＡＮＡＥ）活性、硝基蓝四唑（ＮＢＴ）还原能力及ＣＤ５４表达增强，ＳＴＡＴ３可被ＩＬ－６显著激活，其活性呈一定的剂量与时间依赖性；而ＮＦ－ＩＬ６、ＮＦ－kB、ＡＰ－１及其它ＳＴＡＴ家族成员则无明显激活。结论 Ｕ９３７细胞的终分化可能是ＪＡＫ－ＳＴＡＴ３通路介导的。
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| 关 键 词： | IL－6 U937 STAT3 白细胞因子6 |
| 文章编号： | 1007-8738(2001)01-016-04 |
| 修稿时间： | 2000-01-03 |
IL-6 selectively activatesSTAT3 in U937 cells |
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| ZHANG Ji-yan,LI Yan,SHEN Bei-fen. IL-6 selectively activatesSTAT3 in U937 cells[J]. Chinese journal of cellular and molecular immunology, 2001, 17(1): 16-19 |
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| Authors: | ZHANG Ji-yan LI Yan SHEN Bei-fen |
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| Abstract: | Aim To investigate the signaling basis of the biological effects of IL-6 in U937 cells. Methods We determined the effect of IL-6 on the growth and differentiation of U937 cells and the effect of IL-6 on the activation of nuclear factors in U937 cells. Results IL-6 induced macrophage differentiation of U937 cells. Differentiated cells had strengthened ANAE (acid naphthyl acetate esterase) and NBT(nitroblue tetrazolium) -reducing activities and CD54 expression was upregulated. STAT3 could be remarkably activated by IL-6 in U937 cells and STAT3 activity was dependent on dose and time course of IL-6 treatment. However, NF-IL6, NF-kB, AP-1 and other STAT members were not activated by IL-6. Conclusion IL-6 might induce terminal differentiation of U937 cells via JAK-STAT3 pathway. |
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| Keywords: | IL-6 U937 STAT3 |
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