特发性肺纤维化患者支气管肺泡灌洗液中白细胞介素13的水平及意义

目的　探讨特发性肺纤维化 (IPF)患者支气管肺泡灌洗液 (BALF)和外周血中白细胞介素 13 (IL 13 )水平的变化及其意义。方法　选择 17例IPF患者 (IPF组 )和 8名无器质性肺疾病者 (对照组 )。采用IL 13特异的酶联免疫吸附法测定 (ELISA)法检测 2组BALF和外周血中IL 13的水平 ,分析患者IL 13水平与其肺功能、血气之间的关系。结果　IPF组BALF和外周血中IL 13水平分别为(3 0 1± 86)ng/L、(178± 3 6)ng/L ;对照组分别为 (10 3± 2 4)ng/L、(55± 15)ng/L ,两者比较差异均有显著性 (P <0 0 1)。IPF组BALF中IL 13水平与BALF中性粒细胞数呈正相关 (r =0 786,P <0 0 1) ,与用力肺活量、一秒钟用力呼气容积、肺一氧化碳弥散量及动脉血氧分压均呈一定的等级负相关 (r分别为 -0 898、-0 878、-0 874、-0 890 ,P均 <0 0 1)。结论　IL 13可能在IPF的发病过程中起一定作用 ,并有可能作为判断病变进展情况的一项指标

Evaluation of interleukin-13 in the serum and bronchoalveolar lavage fluid of patients with idiopathic pulmonary fibrosis

OBJECTIVE: To study the significance of interleukin-13 (IL-13) in peripheral blood and bronchoalveolar lavage fluid (BALF) from patients with idiopathic pulmonary fibrosis (IPF). METHODS: The level of IL-13 was determined with ELISA in the serum and BALF of 17 patients with IPF and 8 subjects with non-interstitial lung diseases (non-smokers). RESULTS: The level of IL-13 in the BALF of the patients with IPF was significantly higher than that in non-interstitial lung disease group [(301 +/- 86) ng/L vs. (103 +/- 24) ng/L (P < 0.01)] and was higher than that in serum [(178 +/- 36) ng/L vs. (55 +/- 15) ng/L, P < 0.01]. The level of IL-13 in the BALF of the patients with IPF was positively correlated with the percentage of neutrophils (r = 0.786, P < 0.01). The analysis of Spearman correlation showed that the level of IL-13 in the BALF was correlated with lung function, PaO(2), and D(LCO) (Spearman correlation coefficient r = -0.898, -0.878, -0.874, -0.890, respectively, P < 0.01). CONCLUSION: IL-13 may play an important role in the pathogenesis of IPF and be a potential marker of IPF activity.