| 血管活性肠肽对人脐静脉内皮细胞Ca~(2+)及其膜通道的调控 |
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| 引用本文： | 姚忠祥,蔡文琴.血管活性肠肽对人脐静脉内皮细胞Ca~(2+)及其膜通道的调控[J].解剖科学进展,2002,8(2):137-139. |
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| 作者姓名： | 姚忠祥 蔡文琴 |
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| 作者单位： | 第三军医大学组织学与胚胎学教研室,重庆,40038 |
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| 基金项目： | 国家自然基金资助项目 ( 396 70 382 ) |
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| 摘 要： | 目的 观察血管活性肽 (VIP)对人脐静脉内皮细胞 (HUVEC)膜上Ca2 + 通道及胞浆内游离Ca2 +(Ca2 + ]i)的调控作用。方法 应用共聚焦激光扫描显微术 (CLSM)和膜片钳单通道记录技术对体外培养HU VEC胞浆内 Ca2 + ]i、膜上Ca2 + 通道开放情况进行观察。结果 VIP促使HUVEC胞膜上Ca2 + 通过道的开放概率和胞浆内 Ca2 + ]i 均明显升高。结论 IVP对HUVEC胞浆内 Ca2 + ]i的调节可能除通过细胞内储存的Ca2 +释放外还依赖细胞膜上Ca2 + 通道开放 ,从而达到其调节效应
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| 关 键 词： | 血管活性肠肽 内皮细胞 Ca2+ Ca2+通道 |
| 文章编号： | 1006-2947(2002)02-0137-03 |
| 修稿时间： | 2001年12月13 |
The Regulation on Calcium Ion and Its Channel of Human Umbilical Vein Endothelia Cells by Vasoactive Intestine Peptide |
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| YAO Zhong-xiang,CAI Wen-qin.The Regulation on Calcium Ion and Its Channel of Human Umbilical Vein Endothelia Cells by Vasoactive Intestine Peptide[J].Progress of Anatomical Sciences,2002,8(2):137-139. |
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| Authors: | YAO Zhong-xiang CAI Wen-qin |
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| Abstract: | Objective To observe the regulation on intracytosolic free calcium ion and its membrane channel of human umbilical vein endothelial cells by vasoactiv intestion peptide(VIP).Methods Cibfical laser scanning microscopy and patch-clamp technique were used to observe the concentration of intracytosolic free calcium ion and the opening probability of calcium ion channel in the membrane of human umbilical vein endothelial cells.Results VIP induce the increasing of the concentration of intracytosolic free calcium ion and of the opening probability of calcium ion channel in the cultured human umbilical vein endothelial cells. Conclusion VIP maybe function as the activator of the releasing of the intracellular stored calcium ion, and the inflowing of calcium ion from the outside cell bodies. |
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| Keywords: | vasoactive intestine peptide endothelial cell calcium ion calcium ion channe |
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