| 免疫层析法试剂盒快速鉴别结核与非结核分枝杆菌临床菌株的应用价值 |
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| 引用本文： | 韩敏,乐军. 免疫层析法试剂盒快速鉴别结核与非结核分枝杆菌临床菌株的应用价值[J]. 中国防痨杂志, 2010, 32(5): 57-292 |
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| 作者姓名： | 韩敏 乐军 |
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| 作者单位： | 同济大学医学院附属上海市肺科医院; |
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| 摘 要： | 目的评估免疫层析法试剂盒快速鉴别结核分枝杆菌与非结核分枝杆菌临床菌株的试剂盒应用价值。方法用免疫层析法试剂盒检测240株分枝杆菌菌株和50株其他微生物菌株,包括38株细菌、12株真菌、65株NTM,175株结核分枝杆菌临床菌株。结果结核分枝杆菌株检测阳性为173株,灵敏度为98.9%;115株其他微生物菌株检测结果均为阴性,特异性100%。结论免疫层析法试剂盒能快速、准确鉴别结核与非结核分枝杆菌临床菌株,无需其他设备。
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| 关 键 词： | 分枝杆菌,结核 分枝杆菌属 抗原,细菌 细菌蛋白质类 试剂盒,诊断 |
Evaluation of an Immunochromatographic Assay Kit for Rapid detection of Mycobacterium tuberculosis in Clinical Isolates |
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| Han Min,Yue Jun. Evaluation of an Immunochromatographic Assay Kit for Rapid detection of Mycobacterium tuberculosis in Clinical Isolates[J]. The Journal of The Chinese Antituberculosis Association, 2010, 32(5): 57-292 |
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| Authors: | Han Min Yue Jun |
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| Affiliation: | Shanghai Pulmonary Hospital Affiliated to Tongji University School of Medicine; Shanghai 200433; China; |
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| Abstract: | Objective To evaluate a new immunochromatographic assay(ICA) for rapid discrimination between Mycobacterium tuberculosis and non-tuberculosis mycobacteria(NTM) in clinical isolates. Method 290 clinical isolates including 175 Mycobacterium tuberculosis,65 NTM,38 bacteria,12 fungal isolates were detected using an ICA kit. Results The sensitivity and specificity of the kit were 98.9%(173/175) and 100%(115/115). Conclusions The ICA can detect rapidly and effectively Mycobacterium tuberculosis in clinical isolat... |
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| Keywords: | Mycobacterium tuberculosis Mycobacterium Antigens bacterial Bacterial Proteins reagent kits diagnostic |
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