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| 人脐静脉内皮胞细体外培养及鉴定 | |
| 引用本文： | 李东升,邵素玲,杨万松,黄体钢.人脐静脉内皮胞细体外培养及鉴定[J].临床和实验医学杂志,2012,11(18):1444-1445. |
| 作者姓名： | 李东升 邵素玲 杨万松 黄体钢 |
| 作者单位： | 1. 解放军266医院心内科,河北承德,067000 2. 承德医学院附属医院消毒供应中心,河北,承德,067000 3. 天医科大学第二医院心脏科,天津,300211 |
| 摘 要： | 目的探索人脐静脉内皮细胞体外培养及鉴定的方法。方法采用0.1%Ⅰ型胶原酶分离脐静脉内皮细胞,加入含10%胎牛血清及人AB血清的M1640培养基,在37℃,5%CO2孵箱中培养,以免疫组化方法对内皮细胞进行鉴定。结果原代培养细胞在接种4 h后开始贴壁生长,5～7 d后融合成单层,倒置相差显微镜下观察细胞呈鹅卵石状排列,有接触抑制现象,免疫组化显示细胞胞浆中人Ⅷ因子相关抗原阳性。结论用胶原酶灌注消化脐静脉是获得内皮细胞的一种可靠的方法,有助于体外研究血管内皮细胞模型的构建。 |
| 关 键 词： | 人脐静脉 内皮细胞 细胞培养 鉴定 |
Study on cultivation and identification of hunan umbilical vein endothelial cells in vitro |
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| Institution: | LI Dong - sheng , SHAO Su - ling, YANG Wan - song , et al(. 1. Department of Cardiology, The 266 Hospital of PLA , Chengde Hebei 067000, China ; 2. Central Cterile Supply Department, The Affilated Hospital of Chengde Medical College, Chengde Hebei 067000, China ; 3. Department of Cardiology, Second Hospital of Tianjin Medical University, Tianjin 300211, China. ) |
| Abstract: | Objective To explore the culture method for human umbilical vein endothelial cells（HUVECs） in vitro. Methods HUVECs were obtained with the treatment of type I eoilagenase and cultivated in M1640 medium containing 10% FBS and human AB serum. The ECs were identified by immunohistochemical method under inverted microscopy. Results The primary ceils began to attach and grow after inoculation for 4 hours, and they grew to monolayer after 5 - 7 days. They presented as cobble stone - appearance with contact inhibition under inverted microsco- py, and the examination with antigens of human V$ factor was positive. Conclusion Perfusion with type I coilagenase is an effective way to collect HUVECs in vitro, and it can successfully establish the model of endothelial ceils for research. |
| Keywords: | Human umbilical vein Endothelial cell Cell culture Identification |
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