| 人血浆乙酰水杨酸酯酶动力学特性研究 |
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| 引用本文： | 徐济萍,李军,张鉴,王建筑. 人血浆乙酰水杨酸酯酶动力学特性研究[J]. 中国药学杂志, 2007, 42(16): 1244-1247 |
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| 作者姓名： | 徐济萍 李军 张鉴 王建筑 |
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| 作者单位： | 1. 山东省立医院临床药理中心,济南,250021
2. 山东大学药学院,济南,250021 |
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| 摘 要： | 目的研究中国成年人血浆乙酰水杨酸酯酶的酶动力学特性。方法应用反相HPLC测定培养体系中乙酰水杨酸(ASA)及其主要代谢物水杨酸(SA)的含量,以SA/(ASA+SA)的比值来表示乙酰水杨酸酯酶的活性。结果乙酰水杨酸酯酶的米氏常数Km=429.67nmol·L-1和Vmax=2.56mmol·min-1·L-1(血浆),最佳反应温度为37℃,培养缓冲液pH为7.4。结论该方法灵敏度高、简便、快速、可靠,该酶对pH的变化较敏感,对热极不稳定。
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| 关 键 词： | 乙酰水杨酸酯酶 酶促动力学 高效液相色谱法 人血浆 |
| 文章编号： | 1001-2494（2007）16-1244-04 |
| 收稿时间： | 2006-05-15; |
| 修稿时间： | 2006-05-15 |
Study on Enzyme Kinetics of Aspirin Esterase in Chinese Human Plasma |
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| XU Ji-ping,LI Jun,ZHANG Jian,WANG Jian-zhu. Study on Enzyme Kinetics of Aspirin Esterase in Chinese Human Plasma[J]. Chinese Pharmaceutical Journal, 2007, 42(16): 1244-1247 |
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| Authors: | XU Ji-ping LI Jun ZHANG Jian WANG Jian-zhu |
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| Affiliation: | 1.The Center of Clinical Pharmacology，Shandong Provincial Hospital，Ji’nan 250021，China； 2.School of Pharmaceutics，Shandong University，Ji’nan 250021，China |
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| Abstract: | OBJECTIVE To develop a method for the study of aspirin esterase kinetics in Chinese adult human plasma. METHODS The concentrations of aspirin(ASA)and its metabolites (salicylic acid,SA) were determined by reversed phase HPLC and the aspirin esterase activity was assessed by SA/(ASA+SA).RESULTS The enzyme kinetics analysis revealed that Vmax and Km were 2.56 mmol·min-1·L-1 (plasma) and 429.67 nmol·L-1, respectively. The optimal reaction temperature and pH were 37 ℃ and 7.4, respectively. CONCLUSION The method is simple, specific and reliable, aspirin esterase was sensitive to pH value and heat. |
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| Keywords: | aspirin esterase enzyme kinetics HPLC human plasma |
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