野生型Rb基因转移预防血管成形术后再狭窄的实验研究

目的：探讨人野生型Rb基因转移对预防血管成形术后再狭窄的作用。方法：通过逆转录病毒载体介导，将野生型Rb基因转导至大鼠球囊损伤的颈动脉再狭窄模型中，21天后观察其对新生内膜形成的影响，并检测Rb蛋白表达水平。结果：成功地构建和包装了人野生型Rb基因的重组逆转录病毒载体，并在体外细胞中成功表达；免疫组化检测表明转导血管局部表达人野生型Rb蛋白，与对照组相比，损伤血管新生内膜／中层面积比降低了49％。结论：人野生型Rb基因治疗时血管成形术后再狭窄有一定的预防作用。

Study on preventing restenosis after angioplasty by transferring human wild-type Rb gene

Objective Vascular smooth muscle cell (VSMC) proliferation after injury is important in the pathogenesis of restenosis after balloon angioplasty. In current study,we observed that the effects of human wild-type Rb gene transduction by retroviral vector on preventing restenosis following angioplasty. Methods We constructed and packaged recombinant retrovi ral vector expressing human wild-type Rb. It was transduced into SD rat carotid artery after the establishment of rat carotid balloon injury restenosis model. We measured the neointima/media area ratio and detected human wild-type Rb protein expres sioh in local artery at 21 d after gene transfer. Results We constructed and packaged recombinant retroviral vector expressing human Rb gene successfully. Human wild-type Rb gene was transdued into local carotid artery successfully after rat carotid balloon injury. Human wild-type Rb protein expression was detected in local artery by immunohistochemistry method. Neointima/media area ratio was significantly reduced(49% ) compared with the control group. Conclusion The results suggested a cytostatic gene therapy approach for the restenosis after angioplasty.