| 加热对人肝癌细胞-7721生长规律的影响 |
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| 引用本文： | 齐连君,王执民,张洪新,郭卫平,倪代会. 加热对人肝癌细胞-7721生长规律的影响[J]. 实用放射学杂志, 2000, 16(5): 272-274 |
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| 作者姓名： | 齐连君 王执民 张洪新 郭卫平 倪代会 |
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| 作者单位： | 第四军医大学唐都医院介入放射科,陕西西安 710038 |
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| 摘 要： | 目的观察加热对人肝癌细胞-7721生长规律的影响.方法应用人肝癌细胞株-7721(以下简称7721细胞),常规法培养;采用水浴加温法(42.5℃)热疗和热化疗处理细胞后,继续常规法培养;计数1-7d的细胞数,绘制生长曲线、计算细胞倍增时间;观察细胞的电镜下表现.结果加热处理的细胞倍增时间较未处理细胞明显延长,加热处理的细胞发生空泡变性,热化疗处理的细胞胞膜破坏严重、胞浆细胞器外溢明显.结论加热处理可以明显抑制人肝癌细胞-7721的生长能力,原因是加热引起该细胞的某些细胞器变性.
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| 关 键 词： | 人肝癌细胞-7721 生长曲线 加热 |
| 文章编号： | 1002-1671(2000)05-0272-03 |
| 修稿时间： | 1999-01-13 |
The Effect of Thermotherapy on the Growth law of Human Hpatic Carcinoma Cell-7721 |
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| Abstract: | Objective:To observe the effect of thermotherapy on the growth law of human hepatic carcinoma cell-7721.Methods:To culture the human hepatic carcinoma cell-7721 with routine method,then observe and count the number of cells from 1 to 7 days, draw cells growth curve,calculate cells doubling time,observe the cells exhibition through electron microscope.Results:The doubling time of heated cells is distinctly longer than that of normal cells,the heated cells appear denaturation,the cell membrane and cell organelles and cytoplasm are greatly damaged after thermochemotherapy.Conclusion:The vegetal capability of human hepatic carcinoma cell-7721 is obviously inhbited after they are heated,because some ingredient of cell appear denaturation. [ |
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| Keywords: | human hepatic carcinoma cell-7721 growth carve heat |
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