三七总皂苷对大鼠海马神经细胞缺氧缺糖再给氧损伤的保护作用

目的观察三七总皂苷对大鼠海马神经细胞缺氧缺糖再给氧损伤的保护作用.方法建立缺氧/缺糖再给氧模型,模拟缺血再灌注损伤.流式细胞术检测凋亡细胞百分率,荧光显微镜观察细胞形态学变化和坏死细胞百分率,同时,测定细胞乳酸脱氢酶(LDH)的漏出和一氧化氮(NO)的产生量.结果神经细胞缺氧/缺糖5h后再给氧可诱导神经细胞凋亡和细胞坏死,并显著增加LDH的漏出和NO的产生,并随再给氧时间的延长而增加.三七总皂苷能降低神经细胞凋亡及坏死的百分率,减少LDH的漏出和NO的过量产生,三七总皂苷的作用随剂量增加而增加.结论三七总皂苷具有拮抗海马神经细胞凋亡的作用,这种作用可能与降低或抑制一氧化氮合酶(NOS)活性,减少NO的过量产生有关.

Protective Effects of Panax Notoginseng Saponins on Rat Hippocampal Neuron Injury Induced by Hypoxia/Hypoglycemia and Reoxygenation

Objective:To observe the protective effects of Panax Notoginseng Saponins(PNS) on hypoxia /hypoglycemia and reoxygenation injury of rat hippocampal neurons.Methods: The damaging models of hypoxia /hypoglycemia and reoxygenation were established on cultured rat hippocampal neurons.Apoptosis were measured by flow cytometry.Morphological changes and neuronal necrosis were observed with fluorescence microscope.Nitric oxide(NO) contents and the leakage of lactic dehydrogenase(LDH) were measured.Results: hypoxia/hypoglycemia cultures for 5 hours with reoxygenation afterwards induced neuronal apoptosis and necrosis,significantly increased NO contents and the leakage of LDH.The effect were increased with the extending time of reoxygenation.PNS showed significant decrease in the percentage of neuronal apoptosis and necrosis,and reduced NO contents and the leakage of LDH.Conclusion: PNS may play protectvie role on neuron injury induced by hypoxia /hypoglycemia and reoxygenation by decreasing neuron apoptosis and necrosis,and inhibiting the release of NO and LDH.