10种中药对致病性大肠杆菌O_(86):H_2体外抗菌活性的实验观察

目的:观察黄连、黄芩等10种常见中药的水提取物与醇提取物对致病性大肠杆菌O86:H2的体外抑制作用。方法:选取黄连、黄芩、连翘、大黄、大蒜、三颗针、金银花、白头翁、苦参、马齿苋加水煎煮以及加乙醇浸泡热回流提取法,所得溶液倍比稀释,测定对致病性大肠杆菌O86:H2的最低抑菌浓度(MICs)与最小杀菌浓度(MBC)。结果:连翘、金银花、大黄几乎无抗致病性大肠杆菌活性,其余7种中药显示出不同的抗菌强度,黄连、大蒜、黄芩抗菌活性较好,其中大蒜与黄连乙醇提取物对2株致病性大肠杆菌O86:H2的MICs均<1.96 mg/ml,白头翁、三颗针、马齿苋、苦参的抗菌活性次之。结论:黄连、大蒜与黄芩具有一定的抗致病性大肠杆菌的作用,可从中药材中开发新一代的抗致病性大肠杆菌的药物。

The Experimental Observation of the Inhibitory Effect of Ten Traditional Chinese Medicine (TCM)Preparation on Enteropathogenic E.coil O86:H2 in Vitro

Objective The paper reported some kinds of traditional Chinese medicine's inhibition to Enteropathogenic E.coli,O86:H2,like Coptis chinensis and Scutellaria baicalensis Georgi,etc,including water extractions and alcohol extractions.Methods Choose Coptis chinensis 、Scutellaria baicalensis Georgi 、Fructus Forsythiae 、Allium sativum L、Rheum palmatum L.、Berberis vernae Schneid、Floslonicerae、Radix Pulsatillae、Sophora flavesens、Portulace oleracea L.,put these medicines into boiling water and ethanol,which have been obtained by successive extractions with ethanol,and tested these liquids' MICs and MBC.by broth dilution technique.Results Rheum palmatum L.、Floslonicerae and Fructus Forsythiae are almost no activities against Enteropathogenic E.coli,and the remaining seven kinds of Chinese medicine show a different antibacterial strength,Ccoptis chinensis、Scutellaria baicalensis Georg、Allium sativum L.are good at antibacterial activity.The MICs of Allium sativum and Coptis chinensis with extractions with ethanol are<1.96 mg/ml against 2 strains of Enteropathogenic E.coli O86:H2,the antibacterial activities of Radix Pulsatillae、Berberis vernae Schneid and Sophora flavesens are second.Conclusion Coptis chinensis、Scutellaria baicalensis Georg、Allium sativum L.are bactericidal against Enteropathogenic E.coli,suggesting that Chinese herbal medicines may represent a new source of agents designed for inhibition Enteropathogenic E.coli in eradicated therapy.