Increased expression of growth-associated protein 43 immunoreactivity in axons following compression trauma to rat spinal cord |
| |
Authors: | G L Li M Farooque A Holtz Y Olsson |
| |
Institution: | (1) Laboratory of Neuropathology, University Hospital, S-751 85 Uppsala, Sweden Tel.: 46-18-66 38 38; Fax: 46-18-50 21 72, SE;(2) Department of Neurosurgery, University Hospital, Uppsala, Sweden, SE |
| |
Abstract: | Growth-associated protein 43 (GAP43) is one compound used to indicate growth of axonal endings during development and regeneration,
particularly of peripheral neurons. Using immunohistochemistry, we have studied the expression of GAP43 in the spinal cord
of rats subjected to mild, moderate or severe compression injury and used neurofilament immunostaining to demonstrate axonal
injuries. Samples removed from the compressed T8–9, the cranial T7 and the caudal T10 segments were studied at 4 h, 24 h,
4 days and 9 days after injury. Control rats showed a moderate immunostaining of neurons in dorsal root ganglia, weak staining
of ventral motor neurons and, with the exception of the corticospinal tracts, a weak staining in some axons of the longitudinal
tracts of the cord. Injury in the compressed region led to increased GAP43 immunoreactivity in axons of normal and expanded
size. This occurred particularly 1–4 days after injury and normalized 9 days thereafter. More marked immunostaining was present
in the cranial and caudal segments. The corticospinal tracts never showed such staining. The increase of GAP43 immunostaining
is presumably caused by disturbed axonal transport from neurons with the capacity to synthesize and transport the GAP43 antigen.
Transported material may thus be available for regeneration of axons, but this source of material may vary between different
classes of axons within the cord.
Received: 11 December 1995 / Revised, accepted: 19 January 1996 |
| |
Keywords: | Growth-associated protein 43 Immunohistochemistry Rat Spinal cord Trauma |
本文献已被 SpringerLink 等数据库收录! |
|