Low magnification transmission electron microscopy in diagnostic pathology |
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Authors: | P Rippstein S Cavell M Boivin I Dardick |
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Affiliation: | Department of Laboratory Medicine, Ottawa Civic Hospital, Ontario, Canada. |
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Abstract: | Transmission electron microscopy examination of selected diagnostic specimens, that is, renal and nerve biopsies, can be greatly facilitated by the implementation of slot grid preparations that are inherently devoid of any grid bars. Such preparations are conducive to screening and photography at both low and intermediate modes of magnification without any apparent loss of resolution. The use of this technique in diagnostic electron microscopy is simplified by sequential en bloc staining with both uranyl and lead salts. |
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