A novel factor-dependent human myelodysplastic cell line, MDS92, contains haemopoietic cells of several lineages |
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| Authors: | Kaoru,Tohyama ,Yumi,Tohyama,Takashi,Nakayama ,Takanorf,Ueda ,Toru,Nakamura ,Yataro,Yoshida&dagger |
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| Affiliation: | First Department of Internal Medicine, Fukui Medical School, Matsuoka-cho, Fukui, Japan;Department of Biochemistry, Kobe University School of Medicine, Kobe, Japan;First Division of Internal Medicine, Faculty of Medicine, Kyoto University, Kyoto, Japan |
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| Abstract: | Summary. A novel long-term cultured interleukin(IL)-3-dependent human myelodysplastic cell line, MDS92, was shown to contain several myeloid-lineage cells such as neutrophils, macrophages, eosinophils, and a small number of megakaryocyte-lineage cells. Therefore this cell line possesses at least bipotential characteristics of myeloid- and megakaryocyte-lineages. Granulocyte colony-stimulating factor clearly promoted the neutrophil alkaline phosphatase activity of MDS92 cells. To the contrary, the incidence and growth of CD41-positive cells were hardly affected by the addition of IL-6, IL-11, c-mpl ligand (thrombopoietin, TPO) or erythropoietin. TPO slightly supported the growth of CD34-positive cell fraction, but not CD41-positive cell fraction of MDS92 cells in combination with IL-3 or Steel factor. This cell line will be a useful tool for the study of MDS stem cells, but the mechanism of commitment of differentiation in MDS stem cells remains unknown. |
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| Keywords: | myelodysplastic syndrome factor-dependent cell line multipotential stem cells |
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