| 低频振动对成人成骨细胞增殖分化的影响 |
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| 引用本文： | 江建明,孙炜,冯岚,王吉兴,吴一民,金大地. 低频振动对成人成骨细胞增殖分化的影响[J]. 中国组织工程研究与临床康复, 2002, 6(10): 1418-1419 |
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| 作者姓名： | 江建明 孙炜 冯岚 王吉兴 吴一民 金大地 |
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| 作者单位： | 解放军第一军医大学南方医院,广东广州,510515 |
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| 基金项目： | 广东省自然科学基金资助项目(980948) |
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| 摘 要： | 目的探讨低频振动对成人成骨细胞增殖分化的影响。方法取成人的髂骨松质骨,采用胶原酶-胰蛋白酶联合消化法获得人成骨细胞,进行纯化培养。利用流式细胞仪检测细胞的增殖分化能力。结果不同频率的低频振动对成骨细胞的影响不同。加载0.5Hz振动可使S期的细胞从10.4%增加到16.12%,增殖指数从20.14增加到28.75;2Hz使S期的细胞降低至8.56%,增殖指数降低到17.68%;而5Hz使细胞增殖指数明显降低(P<0.05)。结论适当频率的低频振动能促进成骨细胞增殖分化,对临床应用有积极意义。
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| 关 键 词： | 低频振动 成骨细胞 增殖 分化 |
| 文章编号： | 1671-5926(2002)10-1418-02 |
| 修稿时间： | 2002-03-29 |
The effect of low frequency vibration on cultured human osteoblasts prol iferation and differentiation |
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| JIANG Jian-ming,SUN Wei,FENG Lan,et al.. The effect of low frequency vibration on cultured human osteoblasts prol iferation and differentiation[J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2002, 6(10): 1418-1419 |
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| Authors: | JIANG Jian-ming SUN Wei FENG Lan et al. |
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| Affiliation: | JIANG Jian-ming,SUN Wei,FENG Lan,et al.Nanfang Hospital,Fi rst Military Medical University,Guangzhou 510515,China |
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| Abstract: | Objective To study the effect of low frequency v ibration on cultured human osteobla sts proliferation and differentiation.Methods Osteoblasts isolated form the iliac trabecular bone specimens treated with collagenase-trypsin,and then t he osteoblasts were purified and cultured.Subsequently,The flo w cytomety were used to observe the effect on the proliferation of osteobl asts.Results0.5Hz vibration could increase cell numbers of S phase from 10.4%to 16.12%,PI value from 20.14to 28.75;2Hz reduced PI value to 17.68;5Hz ob-viously decrease PI value(P<0.01).Conclusion0.5Hz vibration could significant a ccelerate the adult human osteoblast proliferation and differentiation. |
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| Keywords: | human low frequency osteoblasts proliferation differentiation |
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