| 高效液相色谱法测定短尾铁线莲中槲皮素的含量 |
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| 引用本文： | 任文栓,白图雅,常福厚. 高效液相色谱法测定短尾铁线莲中槲皮素的含量[J]. 中国民族医药杂志, 2010, 16(2): 50-51 |
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| 作者姓名： | 任文栓 白图雅 常福厚 |
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| 作者单位： | 1. 内蒙古自治区医院,内蒙古,呼和浩特,010017
2. 内蒙古医学院药学院药理学,内蒙古,呼和浩特,010059 |
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| 基金项目： | 内蒙古医学院蒙药现代化研究重大项目资助 |
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| 摘 要： | 目的:测定短尾铁线莲中槲皮素的含量。方法:采用高效液相色谱法,Kromasil(250mm×4.6mm,5μm)色谱柱,甲醇:0.4%磷酸(62:38)为流动相,流速为1.0ml/min;检测波长360nm。结果:槲皮素浓度在21.55μg/ml～63.12μg/ml范围之间与峰面积线性关系良好,r=0.9996。槲皮素平均回收率为101.7%,RSD=1.73%。结论:采用高效液相色谱法测定短尾铁线莲中槲皮素的含量具有分离效果好、简便、结果准确的优点。
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| 关 键 词： | 短尾铁线莲 槲皮素 高效液相色谱法 含量测定 |
Determination of quercetin in Clematis brevicaudata DC by HPLC |
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| Ren Wenquan,Bai Tuya,Chang Fuhou. Determination of quercetin in Clematis brevicaudata DC by HPLC[J]. Journal of Medicine & Pharmacy of Chinese Minorities, 2010, 16(2): 50-51 |
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| Authors: | Ren Wenquan Bai Tuya Chang Fuhou |
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| Affiliation: | 1.Department of.Medicine, Inner Mongolia Hospital, Huhhot 010017, China; 2. Department of pharmacology, Inner Mongolia Medical College, Huhhot 010059, China) |
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| Abstract: | Objective： To determine the content of quercetin in Clematis brevicaudata DC. Methods： a HPLC method was established using Krom sil （250mm × 4.6mm,5μm）with the mobile phase consisting of methanol- 0.4% Phosphoric acid （62：38）,the riot rat was at 1.0ml/min and the UV detection wave length was at 360nm. Results：The calibration curves were liner in the range of 21.55 -63.12μg/ml, r = 0. 9996. The average recovery of quercetin was 101. 7% ,RSD = 1.73%. Conclusion： Determination of quercetin in Clematis brevicaudata DC by HPLC was satisfactory separation effect, simple and accurate. |
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| Keywords: | Clematis brevicaudata DC quereetin HPLC content Determination |
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