| 单克隆抗体ELISA双抗体夹心法测定人白细胞介素2 |
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| 引用本文： | 赵满仓,周景林,刘菊林,黎健,李健斋. 单克隆抗体ELISA双抗体夹心法测定人白细胞介素2[J]. 细胞与分子免疫学杂志, 1992, 0(2) |
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| 作者姓名： | 赵满仓 周景林 刘菊林 黎健 李健斋 |
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| 作者单位： | 空军石家庄医院，空军石家庄医院，空军石家庄医院，卫生部北京老年医学研究所，卫生部北京老年医学研究所 石家庄 050081，石家庄 050081，石家庄 050081 |
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| 摘 要： | 本文叙述了测定人白细胞介素2(IL-2)含量的ELISA双抗体夹心法。用辣根过氧化物酶标记IL-2单克隆抗体。聚苯乙烯反应板预先用0.1％戊二醛处理,然后以单克隆抗体包被。本法批内变异系数CV=3.99％,批间变异系数CV=5.96％,标准曲线的测定范围为3.1～100u/ml,检测下限为1.55u/ml。应用本法已测定了2S例正常人和11例恶性肿瘤患者的IL-2含量。
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| 关 键 词： | 白细胞介素2 ELISA 抗体 单克隆 |
Determination of human interleukin-2 by double antibody sandwich ELISA with monoclonal antibody |
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| Zhao Mancang,Zhou Jinglin,Liu Julin. Determination of human interleukin-2 by double antibody sandwich ELISA with monoclonal antibody[J]. Chinese journal of cellular and molecular immunology, 1992, 0(2) |
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| Authors: | Zhao Mancang Zhou Jinglin Liu Julin |
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| Abstract: | In the present experiment, a double antibody sandwich ELISA method for measurement of human interleukin-2 (IL-2 ) was described. The monoclonal antibody against IL-2 was labelled with horseradish peroxidase. The polystyrene plates were pretreated with 0.1% glutaraldehyde and coated with the monoclonal antibody. Within run and between run variation coefficients of the assay were 3.9% and 5.96%, respectively. The working range of the standard curve for IL-2 was 3. l-100u/ml and the minimal detectable limit was 1.55u/ml. The level of IL-2 in 25 normal subjects and 11 cancer patients were determined using the assay. |
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| Keywords: | interleukin-2 ELISA antibody monoclonal |
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