PCR定量检测肺炎支原体在肺炎支原体肺炎中的应用分析

目的:探讨PCR定量检测在肺炎支原体肺炎诊断中的可行性及优越性。方法:选取186例接受治疗的肺炎患者,分别用MP快速培养法和实时PCR进行检测。记录并比较两组检测方法下的灵敏度及特异性等相关数据。结果:快速培养法与实时PCR法诊断肺炎支原体肺炎的灵敏度、阳性预测值、假阴性率及诊断符合率差异无统计学意义(P>0.05);实时PCR法诊断肺炎支原体肺炎的特异性为85.5%(65/76),明显高于快速培养法的77.6%(59/76),假阳性率为14.5%(11/76),明显低于快速培养法的22.4%(17/76),差异有统计学意义(P<0.05)。结论:快速培养法与实时PCR法诊断肺炎支原体肺炎的敏感度差别不大,而实时PCR法的特异性优于快速培养法。临床上应用这两种不同原理的检测方法组合检测,取长补短,在提高肺炎支原体感染检出率的同时,还可以互相佐证,减少实验误差,提高检测的准确性。

Application Analysis of Quantitative PCR detection of Mycoplasma pneumoniae Mycoplasma pneumoniae pneumonia

Objective To investigate the quantitative PCR in the diagnosis of mycoplasma pneumonia feasibility and superiori- ty. Method 186 cases of patients with pneumonia were treated with MP rapid culture method and the real - time PCR testing respective- ly. the sensitivity and specificity of two methods data were compared. Results The diagnosis of mycoplasma pneumonia sensitivity, positive predictive value, false negative rate and diagnostic accuracy of rapid culture method and real - time PCR had no significant difference （P 〉 0. 05） ;mycoplasma pneumonia diagnosis specificity of Real - time PCR was 85.5% （65/76） , significantly higher than 77.6% of rapid culture （59/76）, the false positive rate was 14.5% （11/76）, significantly lower than the 22.4% of rapid culture （17/76） ,the difference was statistically significant （ P 〈 0. 05）. Conclusion The rapid culture method and real - time PCR diagnosis of mycoplasma pneumonia sensitivity have no different, but the specificity of real - time PCR method is superior to rapid culture method. The clinical application of the principles of these two different combinations can improve the detection rate of Mycoplasma pneumoniae infection, while also supporting each other, to reduce experimental error and improve the accuracy of detection.