| Abstract: | 12 sheep and 4 goats were used to detect the presence of alkaline phosphatase (A.P.) and 5'-nucleotidase (5'-N) enzymes in the carotid rete-cavernous sinus structure. Different methods of preservation were used. The calcium and the lead methods were used to detect the presence of A.P. and 5'-N, respectively. Best results in their detection were obtained with liquid nitrogen preservation. A.P. enzyme was found in and around areas in which blood capillaries were present, indicating active transport of materials through the capillary membrane. Slight enzymatic activity was seen on the endothelial surface of the rete branches, while the enzyme seemed to be absent from the cavernous sinus. 5'-N was discernible in the tunica adventitia and in the endothelial cells, while the tunica media of the rete branches was apparently devoid of this enzyme. Possible role of these enzymes in the vascular wall metabolism of this structure has been discussed. |
