Identification and determination of invertase secreted by tomato cells |
| |
Authors: | Stano J Micieta K Tintemann H Kovács P |
| |
Institution: | Garden of Medicinal Plants, Faculty of Pharmacy, Comenius University, Bratislava, Slovak Republic. Jan.Stano@magist.fpharm.uniba.sk |
| |
Abstract: | A simple and rapid procedure for the identification and determination of extracellular invertase from a culture medium of tomato cell suspension cultures is described. Sucrose was used as substrate for the determination of the extracellular and intracellular activities of the enzyme. The culture medium (without cells) was used for identification and determination of extracellular enzyme activity. Intracellular activity was estimated from the cell suspension. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|