菜豆空间突变品系的分子生物学分析

目的：从分子水平证实经太空飞行处理的菜豆种子后代突变系的遗传物质（ＤＮＡ）确实发生了基因突变。方法：菜豆种子经卫星搭载飞行１５ｄ，回收种植后，采用ＲＡＰＤ的方法对５个叶片形态有变异的突变体的群体及亲本材料进行分析。结果：在５０个１０寡聚核苷酸引物中，有２０个引物扩增出了ＤＮＡ带，共有１８０条带，其大小在２００￣２０００ｂｐ之间，其中有３个引物的扩增产物与对照组相比产生明显的差异。结论：本文首次对空间诱变的菜豆品系进行ＤＮＡ水平上的分析，ＲＡＰＤ分析结果说明该突变品系与原始对照在ＤＮＡ水平上确实已发生了明显变异。

Molecular Analysis of Space Mutant Line of Kidney Bean.

Objective To identify the occurance of gene mutant in mutant lines in the offspring of Kidney bean seeds under space flight condition. Method Kidney bean seeds were carried onboard a recoverable satellite for 15 days in space and were planted on the ground after recovery. Five mutant lines showing variation in the form of leaf blade and their parents were analyzed with RAPD technique. Result 50 random 10-mer primers were used in this study, among which 20 primers generated 180 ploymorphic DNA bands, their size ranged from 200 bp to 2000 bp.3 primers amplified obviously different bands in the DNA of mutant lines in comparison with that of the control. Conclusion This is the first molecular analysis of the mutant lines of Kidney bean generated by space mutagenesis at DNA level. The result of RAPD analysis indicated that distinct variations were demonstrated in the DNA of mutant lines as compared with that of the original control.