Variable toxicity of dyes used in micropuncture studies |
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| Authors: | David J Nikolic-Paterson Lynette A Hurst Wayne D Comper |
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| Affiliation: | Department of Nephrology, Monash University Department of Medicine, Monash Medical Center, and;Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, Australia |
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| Abstract: | SUMMARY: This study evaluated the potential toxicity of Fast green and Lissamine green, the two dyes commonly used for visualizing tubular fluid flow in micropuncture studies. In both the in vitro perfused kidney and cultured tubular epithelial cells, Fast green exhibited a profound, dose-dependent toxicity. In contrast, Lissamine green was non-toxic. In conclusion, we recommend that Fast green not be used in micropuncture studies. |
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| Keywords: | Fast green Lisamine green micropuncture toxicity tubules |
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