| 川芎高效液相指纹图谱的建立 |
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| 引用本文： | 宋金春,胡传芹,刘红,王玉广,李玉琴.川芎高效液相指纹图谱的建立[J].中国药房,2005,16(9):716-718. |
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| 作者姓名： | 宋金春 胡传芹 刘红 王玉广 李玉琴 |
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| 作者单位： | 1. 武汉大学人民医院药学部,武汉市,430060
2. 武汉大学药学院,武汉市,430072 |
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| 摘 要： | 目的建立川芎药材的高效液相指纹图谱,以控制其质量。方法色谱柱为ZorbaxSB-C18,柱温为25℃,流速为1.0ml/min,检测波长为323nm,以甲醇和0.1%冰醋酸水溶液进行梯度洗脱,考察不同提取方式对川芎药材指纹图谱的影响;进行等度洗脱,考察川芎药材在不同波长下的指纹图谱。结果采用超声提取法进行梯度洗脱效果较好。结论本方法可用于建立川芎药材的指纹图谱,且简便、可靠、重现性好。
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| 关 键 词： | 川芎 高效液相色谱法 指纹图谱 质量控制 |
| 文章编号： | 1001-0408(2005)09-0716-03 |
| 修稿时间： | 2004年11月2日 |
Establishment of High Performance Liquid Phase Fingerprint of Chuanxiong |
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| Song Jinchun,HU Chuanqin,LIU Hong,Wang Yuguang,LI Yuqin.Establishment of High Performance Liquid Phase Fingerprint of Chuanxiong[J].China Pharmacy,2005,16(9):716-718. |
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| Authors: | Song Jinchun HU Chuanqin LIU Hong Wang Yuguang LI Yuqin |
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| Abstract: | OBJECTIVE:To establish high performance liquid phase fingerprint for chuanxiong so as to control its quali-ty.METHOD:The chromatographic column was Zorbax SB-C 18 with column temperature at25℃and flow rate at1.0ml/min;the detection wavelength was323nm;the gradient elution was conducted with methanol and0.1%glacial acetic acid water solution.To study the effects of chuanxiong by different extraction methods on fingerprints;the isocratic elution was conducted to investigate the fingerprints of chuanxiong under different detecting wavelength.RESULTS:The ultrasonic method had a good effect in the gradient elution.CONCLUSION:The method is simple,reliable and reproducible,which can be used to es-tablish fingerprint for chuanxiong. |
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| Keywords: | Chuanxiong HPLC Fingerprint Quality control |
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