| 单细胞RNA测序在干细胞生物学中的应用研究进展 |
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| 引用本文： | 肖邦,朱静,徐莎,陈国栋,黄超. 单细胞RNA测序在干细胞生物学中的应用研究进展[J]. 第二军医大学学报, 2023, 44(7) |
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| 作者姓名： | 肖邦 朱静 徐莎 陈国栋 黄超 |
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| 作者单位： | 海军军医大学医学遗传学教研室,海军军医大学中医系中医妇科学教研室,海军军医大学转化医学研究中心干细胞与再生医学研究室,海军军医大学转化医学研究中心干细胞与再生医学研究室,海军军医大学人体解剖学教研室 |
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| 基金项目： | 国家自然科学基金资助项目(82201789),上海市自然科学基金资助项目(22ZR1477000) |
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| 摘 要： | 细胞间的变异或异质性是干细胞群的基本和内在特征。传统的组学分析是在混合细胞群体上进行,导致这些差异被掩盖。近年来,单细胞基因组、表观基因组、转录组测序等技术发展迅速,为全面解析细胞异质性和识别不同表型细胞类型提供了强有利工具。这些方法应用于不同类型的干细胞,包括多能干细胞和组织特异性干细胞,在干细胞领域取得了令人兴奋的新发现。本文综述了单细胞组学测序技术的最新进展,并对单细胞组学测序的方法和应用进行了展望。
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| 关 键 词： | 单细胞RNA测序 干细胞 细胞异质性 |
| 收稿时间： | 2023-04-28 |
| 修稿时间： | 2023-07-06 |
Research progress of application of single cell RNA sequencing in stem cell biology |
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| xiao bang,zhu jing,xu sh,chen guo-dong and huang chao. Research progress of application of single cell RNA sequencing in stem cell biology[J]. Former Academic Journal of Second Military Medical University, 2023, 44(7) |
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| Authors: | xiao bang zhu jing xu sh chen guo-dong huang chao |
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| Affiliation: | The Department of Medical Genetics, Naval Medical University,Department of Gynecology of Traditional Chinese Medicine, Naval Military Medical University,Stem Cell and Regeneration Medicine Institute, Research Center of Translational Medicine, Naval Medical University,Stem Cell and Regeneration Medicine Institute, Research Center of Translational Medicine, Naval Medical University,Department of Anatomy, Institute of Biomedical Engineering, Naval Medical University |
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| Abstract: | Cell-to-cell variation and heterogeneity are fundamental and intrinsic characteristics of stem cell populations, but these differences are masked when bulk cells are used for traditional omic analysis. These technologies, including single-cell genome, epigenome, and transcriptome sequencing technologies, have been developing rapidly in recent years. Single-cell sequencing technologies serve as powerful tools to dissect cellular heterogeneity comprehensively and to identify distinct phenotypic cell types. The application of these methods to different types of stem cells, including pluripotent stem cells and tissue-specific stem cells, has led to exciting new findings in the stem cell field. In this review, we discuss the recent progress as well as future perspectives in the methodologies and applications of single-cell omic sequencing technologies. |
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| Keywords: | Single-cell RNA sequencing Stem cells cells heterogeneity |
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