Early Effects Triggered by Larrea divaricata Cav. on Murine Macrophages at Apoptotic Concentrations |
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| Authors: | Roberto Davicino Aida Mattar Yolanda Casali Carina Porporatto Silvia G Correa Blas Micalizzi |
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| Institution: | 1. Area Microbiología, Departamento de Bioquímica y Ciencias Biológicas, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis. Chacabuco y Pedernera, San Luis, Argentina;2. Area Bromatología y Ensayo y Valoración de Medicamentos, Departamento de Farmacia, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, San Luis, Argentina;3. Inmunología, CIBICI Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina |
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| Abstract: | Decoction and infusion of Larrea divaricata were tested at apoptotic concentrations (1 and 4 mg/ml) on peritoneal murine macrophages. Consistent changes were observed after incubation with 4 mg/ml decoction. Phagocytosis of zymosan, lysosomal enzyme activity, nitric oxide production, TNF-α release, and expression of CD14, TLR4, and CR3 increased significantly. Decoction at 1 and 4 mg/ml increased the binding of LPS-FITC. Apoptosis triggered by L. divaricata decoction is consequence of cell activation. The effects are independent of nordihydroguaiaretic acid. This “activation and death” could be the mechanism of L. divaricata to exert the antituberculosis effect known in folk medicine. |
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| Keywords: | Decoction Larrea divaricata Macrophages Mycobacterium tuberculosis |
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