Separation Methods of T Cells,Natural Killer,and Dendritic Cells from Peripheral Blood of Cancer Patients using Interleukin-2 and Functional Analysis of Natural Killer Cells after Separation |
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| Authors: | Norimasa Matsushita Atsushi Aruga Yasunobu Kobayashi Keishi Tanigawa Masakazu Yamamoto |
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| Institution: | 1. Cancer Immunotherapy and Cell Therapy, Department of Surgery, Graduate School of Medicine and Department of Gastroenterological Surgery, Tokyo Women's Medical University, Tokyo, Japan;2. J.B. Therapeutics, Tokyo, Japan;3. Department of Gastroenterological Surgery, Tokyo Women's Medical University, Tokyo, Japan |
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| Abstract: | We aimed to induce three different immune cell subsets from a single blood sample from cancer patients to target different biological characters of cancer cells. In the presence of 6000 IU/ml IL-2, natural killer (NK) cells adhere to plastic. By using this ability, we could separate dendritic cells, T cells, and NK cells from peripheral blood mononuclear cells. The cultured NK cells demonstrated higher nonspecific cytotoxicity against tumor cell lines than did the T cells. Furthermore, adherent NK cells demonstrated higher cytotoxicity than nonadherent NK cells, although there was no difference between adherent and nonadherent NK cells in natural cytotoxicity receptors (NKp30, NKp44, NKp46) and NKG2D expression. With these results, we confirmed that we could induce dendritic cell, T cell, and higher cytotoxic NK cells from a single blood draw, and this methodology facilitates to the use of these cells for clinical grade conditions. |
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| Keywords: | Adherent NK Cell CD3-Activated T Cell Immunotherapy Interleukin-2 Natural Cytotoxicity Receptor |
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