肾癌组织的端粒酶活性

目的 :探讨端粒酶活性与肾细胞癌之间的关系。方法 :采用改良TRAP法对 32例肾细胞癌组织及正常肾组织和可疑癌旁组织中端粒酶活性进行检测 ,同时了解其与临床生物学特征的关系。结果 :32例肾细胞癌组织中端粒酶强阳性 17例 ,阳性 12例 ,阴性 3例 ,阳性率 91% ;32例正常肾组织中端粒酶弱阳性者仅 2例 ,阳性率 6 % ;可疑癌旁组织阳性者 6例 ,阳性率 19%。三组端粒酶活性比较 ,阳性率差异有显著性 (P <0 0 1)。结论 :肾细胞癌组织中端粒酶活性检出率高 ,特异性强 ,无假阳性和假阴性。提示端粒酶活检测可作为判断肾肿瘤恶性程度的标记物 ,并可能成为判断肾肿瘤预后的一项指标

Telomerase Activity in Human Renal Cell Carcinoma with Reference to Clinicopathologic Features

Objective: To study the telomerase activity in human renal cell carcinoma and to evaluate the correlation with the clinicobiologic features of the neogrowth.Methods: The telomerase activity was studied by means of a modified telomeric repeat amplification protocol (TRAP) in 32 renal cell carcinoma tissues, 32 normal renal tissues and 32 paracancer tissues and its correlation with the clinicopathologic features of the tumor was evaluated.Results: Telomerase activity was strongly positive in 17, positive in 12 and negative in 3 cases of renal cell carcinoma tissues, the total positive rate being 91%. Telomerase activity was weakly positive (6%) in only 2 out of 32 samples of normal renal cortex tissues and positive in 6 paracancer tissues (19%), the difference was conspicuous (P<0.01).Conclusion: The positive rate of telomerase activity was significantly higher in renal cell carcinoma tissues and might serve as a prognostic marker for estimating the biologic characteristics of renal cell carcinoma.