地氟醚预处理对缺氧/复氧损伤内皮细胞内游离Ca~(2+)浓度和钙网蛋白表达的影响 |
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引用本文: | 李渊,仓静,薛张纲.地氟醚预处理对缺氧/复氧损伤内皮细胞内游离Ca~(2+)浓度和钙网蛋白表达的影响[J].临床麻醉学杂志,2009,25(11). |
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作者姓名: | 李渊 仓静 薛张纲 |
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作者单位: | 复旦大学附属中山医院麻醉科,上海市,200032 |
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基金项目: | 2006年复旦大学青年科学基金 |
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摘 要: | 目的 观察地氟醚预处理对缺氧/复氧(A/R)损伤的内皮细胞内游离Ca~(2+)浓度及钙网蛋白表达水平的影响.方法 人脐静脉内皮细胞株(ECV304)细胞分为三组:A/R组(Ⅰ组)、地氟醚1.0 MAC预处理+A/R组(Ⅱ组)和空白对照组(Ⅲ组).应用Fluo-3/AM荧光探针法和Western blot方法检测细胞中游离Ca~(2+)浓度和钙网蛋白水平.结果 与Ⅲ组比较,Ⅰ和Ⅱ组细胞中游离Ca~(2+)浓度显著增高(P<0.01),但Ⅱ组的升高幅度明显低于Ⅰ组(P<0.05);Ⅱ组细胞中游离Ca~(2+)负载有所增高,但钙网蛋白表达水平增加(P<0.05).结论 地氟醚预处理可通过降低细胞内的钙超载来减轻A/R造成的损伤.
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关 键 词: | 地氟醚 预处理 缺氧/复氧损伤 钙超载 钙网蛋白 |
Effects of desflurane preconditioning on intracellular free Ca~(2+) and calreticulin expression in the endothelial cells underwent anoxia/reoxygenation |
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Abstract: | Objective To observe the effects of desflurane preconditioning on the intracellular free Ca~(2+) and calreticulin expression in the endothelial cells Ⅰ underwent anoxia/reoxygenation (A/R).Methods Human umbilical vein endothelial cells(ECV304)were divided into three groups of A/R(group Ⅰ),Des + A/R(group Ⅱ)and control(groupⅢ).The concentrations of free Ca~(2+) were measured with Fluo-3/AM florescent probe and the expressions of calreticulin(CRT)protein were detected with Western blot.Results Compared with group Ⅲ,the concentration of free Ca~(2+) was higher and the expression of CRT was lower in group Ⅰ(P<0.01),which were opposite in group Ⅱ (P<0.05).Conclusion Desflurane preconditioning can protect the endothelial cells by attenuating the Ca~(2+) overload caused by A/R injury. |
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Keywords: | Desflurane Preconditioning Anoxia/reoxygenation Ca~(2+) overload Calreticulin |
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