椰毒假单胞菌酵米面亚种16S rDNA序列测定与分析

本文对３种不同来源样品的４株椰酵假单胞菌的１６ＳｒＤＮＡ的序列进行了测定，以确定其系统发育位置及与其它菌种的遗传进化关系。实验中设计了７条引物，利用聚合酶链式反应（ＰｏｌｙｍａｓｅＣｈａｉｎＲｅ－ａｃｔｉｏｎ，ＰＣＲ）扩增４株细菌的１６ＳｒＤＮＡ，通过扩增产物的克隆测序或直接测序，获得了它们的１６ＳｒＤＮＡ序列，将测得的序列与伯克霍尔德氏菌属中其它菌种的１６ＳｒＤＮＡ序列进行了聚类分析，得到了系统发育进化树状图。树状图及不同菌种之间的同源性比值：椰酵假单胞菌（Ｐ．ｃｏｃｏｖｅｎｅｎａｎｓｓｕｂｓｐ．ｆａｒｉｎｏｆｅｒｍｅｎｔａｎｓ）与唐菖蒲伯克霍尔德氏菌（Ｂｕｒｋｈｏｌｄｅｒｉａｇｌａｄｉｏｌｉ）、椰毒伯克霍尔德氏菌（Ｂｕｒｋｈｏｌｄｅｒｉａｃｏｃｏｖｅｎｅｎａｎｓ）的亲缘关系非常近，与其它菌种的亲缘关系相对较远，椰酵假单胞菌、唐菖蒲伯克霍尔德氏菌和椰毒伯克霍尔德氏菌可归属于一个独立的系统发育系。

Squencing and analysis of 16S rDNA sequences for P.cocovenenans subsp farinofermentans

In order to fix the phylogenetic taxonomic position of P. cocovenenans subsp.farinofermentans and describe its relationships with other closed species,seven primers were designed to amplify and sequence the 16S rDNA of 4 strains of it by using a clonal sequencing or direct sequencing method.The 16S rDNA sequence of the 4 strains were aligned with the 16S rDNA sequences of other species in genus Burkholderia ,and the polygenetic tree was produced by using a Clustal V and treeview software.The results showed that P. cocovenenans subsp.farinofermentans was in a high homology with Burkholderia gladioli and Burkholderia cocovenenans , and they formed an independent phylogenetic clustal of genus Burkholderia.