| 内皮祖细胞促动脉内皮损伤修复的研究 |
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| 引用本文： | 司逸,符伟国,竺挺,董智慧,张祥满,王玉琦. 内皮祖细胞促动脉内皮损伤修复的研究[J]. 中国分子心脏病学杂志, 2010, 10(1): 17-22 |
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| 作者姓名： | 司逸 符伟国 竺挺 董智慧 张祥满 王玉琦 |
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| 作者单位： | 复旦大学附属中山医院血管外科,上海,200032 |
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| 基金项目： | 高等学校博士学科点专项科研基金，复旦大学生物医学研究院开放课题研究基金 |
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| 摘 要： | 目的评价骨髓源性内皮祖细胞(progenitor endothelial cells,EPCs)移植促动脉损伤内皮修复的效果。方法将菲立磁(superparam agnetic iron oxide,SPIO)和绿色荧光蛋白(green fluorescent protein,GFP)双标记的EPC局部注入损伤颈动脉段腔内。术后1d、7d和28d行磁共振(magnetic resonance imaging,MRI)扫描、组织学检查及内皮型一氧化氮合酶(endothelial nitric oxide synthase,eNOS)检测。结果MRI扫描发现移植部位出现低信号区。移植组HE、普鲁士蓝、vWF免疫组化染色、GFP荧光、偶氮蓝(Evans blue)染色均提示EPC可粘附于内膜损伤处,并随时间分化为内皮细胞;同时eNOS表达随时间变化而增多。结论菲立磁标记内皮祖细胞可促进动脉损伤内皮的修复,并可为MRI示踪。
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| 关 键 词： | 内皮祖细胞 内皮损伤 磁共振 内皮型一氧化氮合酶 普鲁士蓝 免疫组化染色 移植 内皮细胞 细胞粘附 菲立磁 颈动脉 |
Restore Rabbit Injuried Arterial Intima by Bone Marrow Derived Progenitor Endothelial Cell Transplantation |
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| SI Yi,FU Wei-guo,ZHU Ting,DONG Zhi-hui,ZHANG Xiang-man,WANG Yu-qi. Restore Rabbit Injuried Arterial Intima by Bone Marrow Derived Progenitor Endothelial Cell Transplantation[J]. Molecular Cardiology of China, 2010, 10(1): 17-22 |
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| Authors: | SI Yi FU Wei-guo ZHU Ting DONG Zhi-hui ZHANG Xiang-man WANG Yu-qi |
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| Affiliation: | SI Yi,FU Wei-guo,ZHU Ting,DONG Zhi-hui,ZHANG Xiang-man,WANG Yu-qi. Department of Vascular Surgery,Zhongshan Hospital,Fudan University,Shanghai 200032,China |
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| Abstract: | Objective To survey the effects of the bone marrow derived progenitor endothelial cells (EPCs) of repairing arterial intimal injury.Methods EPCs,labeled by the superparamagnetic iron oxide (SPIO) and green fluorescent protein (GFP),were injected into the injury artery lumen.At 1d,7d and 28d after cell transplantation,magnetic resonance imaging (MRI),HE,Prussian blue staining,biopsy,immunohistochemistry,immunofluorescence and western blot for endothelial nitric oxide synthase (eNOS) were applied to evaluate ... |
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| Keywords: | Progenitor endothelial cell Intimal injury Magnetic resonance imaging eNOS |
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