Detection of rpoB mutations in Mycobacterium tuberculosis by biprobe analysis |
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| Authors: | Edwards K J Metherell L A Yates M Saunders N A |
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| Affiliation: | Molecular Biology Unit, Virus Reference Division, Central Public Health Laboratory, Colindale, London, NW9 5HT, United Kingdom. |
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| Abstract: | A biprobe assay utilizing LightCycler technology was developed to detect rifampin resistance-associated gene mutations in the Mycobacterium tuberculosis rpoB gene. Three biprobes detected all mutations present in the 46 rifampin-resistant isolates. Wild-type sequences were correctly identified in each case. The method was reproducible, accurate, and easy to use. |
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