高效液相色谱法测定明目地黄丸（浓缩丸）中丹皮酚含量

目的建立测定明目地黄丸（浓缩丸）中丹皮酚含量的反相高效液相色谱法。方法采用Agilent Zorbax SB C18（250 mm×4.6 mm，5 μm）色谱柱；流动相为甲醇-水（48：52）；检测波长为274 nm；流速为1.0 mL•min－1；外标法计算含量。结果丹皮酚在32.06～1 282.40 ng范围内呈良好的线性关系（r＝1.000 0），平均回收率96.38%，RSD为0.80%（n＝6）。结论该方法前处理简便、准确，灵敏度高，重复性好，可作为该制剂的质量控制方法之一。

Determination of Paeonol in Mingmu Dihuang Pills (Concentrated Pills) by HPLC

Objective To establish a method for the determination of paeonol in mingmu dihuang pills(concentrated pills)by RP-HPLC.Methods The analysis was performed on a Agilent Zorbax SB-C18 column(250 mm×4.6 mm,5 μm) with methanol-water(48:52) as mobile phase,at a flow rate of 1.0 mL·min-1.The detection wavelength was at 274 nm.The content was calculated with external standard.Results The linear range of paeonol was good within 32.06-1 282.40 ng(r=1.000 0).The average recovery was 96.38%,with RSD as 0.80%(n=6).Conclusion This method is simple,accurate,sensitive,repeatable and can be used for quality control of the preparation.