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鼻咽癌细胞株中标记滞留细胞(LRGs)的建立与检测(英文)
引用本文:江庆萍,姚开泰.鼻咽癌细胞株中标记滞留细胞(LRGs)的建立与检测(英文)[J].癌症,2010(5).
作者姓名:江庆萍  姚开泰
作者单位:Cancer Institute;Basic Medicine;Southern Medical University;Department of Pathology;The Third Affiliated Hospital;Guangzhou Medical University;
摘    要:

关 键 词:标记  滞留  鼻咽癌细胞株  检测  

Isolation and detection of label-retaining cells in a nasopharyngeal carcinoma cell line
Qing-Ping Jiang,Kai-Tai Yao Cancer Institute,Basic Medicine,Southern Medical University,Guangzhou,Guangdong ,P.R.China.Isolation and detection of label-retaining cells in a nasopharyngeal carcinoma cell line[J].Chinese Journal of Cancer,2010(5).
Authors:Qing-Ping Jiang    Kai-Tai Yao Cancer Institute  Basic Medicine  Southern Medical University  Guangzhou  Guangdong  PRChina
Institution:Qing-Ping Jiang1,2,Kai-Tai Yao1 1 Cancer Institute,Basic Medicine,Southern Medical University,Guangzhou,Guangdong 510515,P.R.China,2 Department of Pathology,The Third Affiliated Hospital,Guangzhou Medical University,Guangdong 510150
Abstract:Background and Objective:Detection of label-retaining cells(LRCs) has been a method to confirm existence of stem cells,and bromodeoxyuridine(BrdU) has commonly been used for labeling.In this study,to verify stem cells in nasopharyngeal carcinoma(NPC),LRCs were established and detected in NPC cell line 5-8F.Methods:The 5-8F cells were cultured with BrdU and inoculated subcutaneously into nude mice.By immunohistochemistry,immunocytochemistry,and immunofluorescence,BrdU was detected in 5-8F cells and xenograft...
Keywords:Label-retaining cells(LRCs)  nasopharyngeal neoplasm  BrdU  stem cells  
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