复方除湿洗液抗炎作用研究

目的研究复方除湿洗液抗炎作用及作用机制。方法采用二甲苯致小鼠耳肿胀模型、角叉菜胶致小鼠足肿胀模型,分析天平称量左右耳片质量、左右后足质量,观察不同浓度复方除湿洗液对小鼠炎症的影响;紫外分光光度法检测足肿胀组织前列腺素E2含量。结果复方除湿洗液低剂量组（FL组）、中剂量组（FM组）和阳性对照组（C组）小鼠左右耳质量差［（0.009 3±0.003 6）,（0.011 8±0.003 1）和（0.012 1±0.003 4）g］均小于模型组［M组,（0.015 5±0.002 9）g,P＜0.01或P＜0.05］;FL组、FM组、FH组左右足质量差［分别为（0.026 0±0.011 0）,（0.024 4±0.013 8）,（0.015 6±0.007 5） g］均低于M组［（0.039 5±0.019 5）,P＜0.01或P＜0.05］,肿胀足组织前列腺素E2（A/g）［（0.494 0±0.044 5）,（0.509 1±0.036 7）,（0.491 0±0.076 6）］均低于M组［（0.575 7±0.069 7）,P＜0.01或P＜0.05］。结论复方除湿洗液具有明显抗炎作用,其抗炎作用机制可能与抑制PGE2产生有关。

Anti-inflammatory Effects of Compound Chushi Lotion

Objective To investigate the anti-inflammatory effects and possible mechanisms of compound chushi lotion.Methods The anti-inflammatory effects of compound chushi lotion were studied on the mice models of ear flaps swollen induced by dimethylbenzene and toes swollen induced by carrageenan,via weighing both earlaps and toes of mice and detecting prostaglandin E2 in the swollen toes with ultraviolet spectrophotometer.Results The weight difference between left and right earlaps in the low-dose group of compound chushi lotion,middle-dose group,and positive control[C,(0.012 1±0.003 4) g] was less obvious than that of the model control[M,(0.015 5±0.002 9) g](P<0.01 or P<0.05).The mass difference between left toes and right ones of FL、FM and FH groups was(0.026 0±0.011 0),(0.024 4±0.013 8),(0.015 6±0.007 5) gram,respectively,which was slighter than that in the model group[(0.039 5±0.019 5),P<0.01 or P<0.05].The prostaglandin E2(absorbance/gram) in swollen toes of these groups was(0.494 0±0.044 5),(0.509 1±0.036 7) and(0.491 0±0.076 6),respectively,which was lower than the model group[(0.575 7±0.069 7),P<0.01or P<0.05].Conclusion Compound chushi lotion has anti-inflammatory effect,the mechanism of which is possibly associated with inhibition of prostaglandin E2.