小分子干扰RNA抑制肺癌细胞Survivin表达对凋亡和顺铂耐药性的影响

目的 观察应用小分子干扰RNA(siRNA)技术抑制肺癌A549细胞中Survivin表达对细胞凋亡和顺铂耐药性的影响.方法 设计、合成特异性抑制Survivin表达的siRNA,转染肺癌A549细胞48 h后,检测Survivin基因mRNA表达量以及肺癌细胞凋亡率和对顺铂耐药性变化.结果 A549细胞转染Survivin特异siRNA后,Survivin/β-actin基因mRNA表达比例1.17±0.25下降至0.41±0.18,抑制率65.10%;细胞凋亡率由2.67%上升至32.33%;顺铂半数抑制浓度(ICSO)由6.37 ms/L降低至2.42 ms/L.结论 通过siRNA特异性抑制肺癌A549细胞中Survivin基因表达可增加凋亡,逆转顺铂耐药.

Silencing survivin expression by siRNA in lung cancer cell enhanced apoptosis and reversed cispla tin resistance

Objective To evaluate the effect of survivin siRNA silencing survivin gene on the apoptosis rate and sensitivity to cisplatin of lung cancer A549 cells.Methotis A549 cells were transfected with survivin siRNA for 48 h.Survivin mRNA expression.apoptosis rate and sensitivity to cisplatin were checked.Reslllts After survivin-siRNA transfection.survivin expression was decreased from 1.17±0.25 to 0.41±0.18 shown as survivin/β-actin mRNA ratio,and apoptosis rate increased from 2.67%to 32.33%,and IC50 to cisplatin decreased from 6.37 t0 2.42 ms/L.Conclusion Survivin gene silencing by specific siRNA in lung cancer A549 cells Can induce apoptosis and sensitize cells to cisplatin.