Generation and evaluation of an H9N1 influenza vaccine derived by reverse genetics that allows utilization of a DIVA strategy for control of H9N2 avian influenza |
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Authors: | Rui Wu Quanjiao Chen Liyun Zheng Jianjun Chen Zhiwei Sui Yi Guan and Ze Chen |
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Institution: | (1) State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, 430071 Wuhan, Hubei, China;(2) Graduate University of Chinese Academy of Sciences, 100049 Beijing, China;(3) Department of Microbiology, University of Hong Kong, Hong Kong Special Administrative Region, Pokfulam, China;(4) College of Life Sciences, Hunan Normal University, 410081 Changsha, Hunan, China;(5) Shanghai Institute of Biological Products, 200052 Shanghai, China |
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Abstract: | H9N2 avian influenza viruses have circulated widely in domestic poultry around the world, and their outbreaks have resulted
in heavy morbidity and mortality. In addition, H9N2 avian influenza viruses were transmitted directly from birds to humans
in Hong Kong and mainland China during 1998 and 2003, which prompted the public health authorities to seek protective strategies
to control H9N2 influenza viruses. In this study, we attempted to develop a DIVA (differentiating infected and vaccinated
animals) strategy for H9N2 avian influenza viruses. This strategy does not interfere with serological monitoring and allows
effective control of H9N2 avian influenza. We generated a reassortant H9N1 influenza vaccine strain by reverse genetics and
employed an enzyme-linked immunosorbent assay (ELISA) with a truncated N1 antigen expressed in E. coli to differentiate between vaccinated and naturally infected animals. Immunization of BALB/c mice with the inactivated reassortant
H9N1 vaccine conferred protection against lethal challenge with H9N2 viruses. Meanwhile, the ELISA can be used to distinguish
between vaccination and natural infection quickly and easily. Therefore, this study has opened up a new avenue for the control
of H9N2 avian influenza. |
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